High-efficiency cell disruption and astaxanthin recovery from Haematococcus pluvialis cyst cells using room-temperature imidazoliumbased ionic liquid/water mixtures
- Authors
- Choi, Sun-A; Oh, You-Kwan; Lee, Jiye; Sim, Sang Jun; Hong, Min Eui; Park, Ji-Yeon; Kim, Min-Sik; Kim, Seung Wook; Lee, Jin-Suk
- Issue Date
- 2월-2019
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Haematococcus pluvialis cyst; Ionic liquid; Astaxanthin extraction; Lipid extraction; Cell disruption
- Citation
- BIORESOURCE TECHNOLOGY, v.274, pp.120 - 126
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 274
- Start Page
- 120
- End Page
- 126
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/67808
- DOI
- 10.1016/j.biortech.2018.11.082
- ISSN
- 0960-8524
- Abstract
- Energy-saving, high-efficiency cell disruption is a critical step for recovery of thermolabile antioxidant astaxanthin from Haematococcus pluvialis cyst cells of rigid cell-wall structure. In this study, as room-temperature green solvents, 10 types of 1-ethyl-3-methylimidazolium([Emim])-based ionic liquids (ILs) were compared and evaluated for their abilities to disrupt H. pluvialis cyst cells for astaxanthin/lipid extraction. Among the 10 ILs tested, 3 [Emim]-based ILs with HSO4, CH3SO3, and (CF3SO2)(2)N anions were selected based on astaxanthin/lipid extraction performance and synthesis cost. When pretreated with IL/water mixtures, intact cyst cells were significantly torn, broken or shown to release cytoplasmic components, thereby facilitating subsequent separation of astaxanthin/lipid by hexane. However, excess IL pretreatments at high temperature/IL dosages and longer incubation times significantly deteriorated lipid and/or astaxanthin. Under optimized mild conditions (6.7% (v/v) IL in water solution, 30 degrees C, 60 min), almost complete astaxanthin recoveries (> 99%) along with moderate lipid extractions (similar to 82%) could be obtained.
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Collections - College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles
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