One-step multiplex real-time RT-PCR for detection and typing of dengue virus
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mun, Myung-Jin | - |
dc.contributor.author | Bae, Joon-Yong | - |
dc.contributor.author | Kim, Jin Hyuck | - |
dc.contributor.author | Kim, Soo Bok | - |
dc.contributor.author | Lee, Ilseob | - |
dc.contributor.author | Kim, Jin Il | - |
dc.contributor.author | Park, Mee Sook | - |
dc.contributor.author | Park, Man-Seong | - |
dc.contributor.author | Nam, Yong Suk | - |
dc.date.accessioned | 2021-09-01T20:10:23Z | - |
dc.date.available | 2021-09-01T20:10:23Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-02 | - |
dc.identifier.issn | 0890-8508 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/67840 | - |
dc.description.abstract | Previous studies reported that severity of dengue is associated with multiple factors, including secondary infection, age, viral load and infecting serotype and genotype. In addition, other studies have reported that a dengue virus-2 (DENV-2) infection is associated with a prognosis of more severe clinical manifestations than DENV-1 and DENV-4 infections. For these reasons, the ability to identify the DENV serotypes is critical for optimal patient diagnosis and epidemiological studies. In this study, we developed a TaqMan probe-based, onestep real-time reverse transcriptase-polymerase chain reaction (RT-PCR) system for detection and serotyping DENV. Our linear dynamic range (10(1) to 10(7) copies/reaction) showed the R-2 values of DENV-1, 2, 3 and 4 as 0.998, 0.998, 0.994, and 0.998, respectively. The detection limits of DENV-1, 2, 3, and 4, were 10 copies/reaction, 100 copies/reaction, 10 copies/reaction, and 100 copies/reaction, respectively. Specificity test results indicated that this system is specific for DENV-1, 2, 3, and 4 and does not react with other viruses. Finally, we validated our results with five different real-time PCR instruments. Our results showed that the Ct values of the four serotype templates were similar in five real-time PCR instruments. Thus, this system provides an accurate method for detection and serotyping of DENV, which can be applied in diagnostics, surveillance, and epidemiology. Dengue can be found in many nations with varying socioeconomic and monetary resources. The results of our validation analyses using five different real-time PCR instruments suggest that this method can easily and confidently be used world-wide. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | - |
dc.subject | SEROTYPE-SPECIFIC DIFFERENCES | - |
dc.subject | CHIKUNGUNYA | - |
dc.subject | ASSAYS | - |
dc.title | One-step multiplex real-time RT-PCR for detection and typing of dengue virus | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jin Il | - |
dc.contributor.affiliatedAuthor | Park, Man-Seong | - |
dc.identifier.doi | 10.1016/j.mcp.2018.10.001 | - |
dc.identifier.scopusid | 2-s2.0-85056328484 | - |
dc.identifier.wosid | 000457350700015 | - |
dc.identifier.bibliographicCitation | MOLECULAR AND CELLULAR PROBES, v.43, pp.86 - 91 | - |
dc.relation.isPartOf | MOLECULAR AND CELLULAR PROBES | - |
dc.citation.title | MOLECULAR AND CELLULAR PROBES | - |
dc.citation.volume | 43 | - |
dc.citation.startPage | 86 | - |
dc.citation.endPage | 91 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | SEROTYPE-SPECIFIC DIFFERENCES | - |
dc.subject.keywordPlus | CHIKUNGUNYA | - |
dc.subject.keywordPlus | ASSAYS | - |
dc.subject.keywordAuthor | Dengue virus | - |
dc.subject.keywordAuthor | Serotyping | - |
dc.subject.keywordAuthor | TaqMan probe | - |
dc.subject.keywordAuthor | Real-time PCR | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.