Effects of gltA and arcA Mutations on Biomass and 1,3-Propanediol Production in Klebsiella pneumoniae
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jung Hun | - |
dc.contributor.author | Jung, Hwi-Min | - |
dc.contributor.author | Jung, Moo-Young | - |
dc.contributor.author | Oh, Min-Kyu | - |
dc.date.accessioned | 2021-09-01T20:20:48Z | - |
dc.date.available | 2021-09-01T20:20:48Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-02 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/67881 | - |
dc.description.abstract | We have previously engineered a Klebsiella pneumoniae strain to increase the 1,3-production (1,3-PDO) yield from glycerol. Here, we describe the further engineering of this strain to improve the biomass formation, resulting in an increase in the 1,3-PDO production. The amino acid lysine at the 167th position in citrate synthase was substituted with alanine using genome editing method to reduce the binding affinity of the enzyme to nicotinamide adenine dinucleotide (NADH). In addition, the arcA gene was deleted that resulted in the inhibition of the expression of citric acid cycle genes under limited aeration conditions. As a consequence, the biomass production was enhanced by 34% and 1,3-PDO formation was elevated from 9.58 to 16.71 g/L. The production of 1,3-PDO per dry cell weight enhanced by 30% from 2.40 to 3.11 gL(-1)DCW(-1). The phenotypic changes in the strains were confirmed through the analyses of redox ratio, ATP levels, and changes in the expression of genes related to citric acid cycle and 1,3-PDO pathway. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.subject | NADH BINDING-SITE | - |
dc.subject | CITRATE SYNTHASE | - |
dc.subject | TRANSCRIPTIONAL REGULATION | - |
dc.subject | TCA CYCLE | - |
dc.subject | EXPRESSION | - |
dc.subject | GLYCEROL | - |
dc.subject | SEQUENCE | - |
dc.subject | CRISPR | - |
dc.subject | GENES | - |
dc.subject | FNR | - |
dc.title | Effects of gltA and arcA Mutations on Biomass and 1,3-Propanediol Production in Klebsiella pneumoniae | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
dc.identifier.doi | 10.1007/s12257-018-0246-0 | - |
dc.identifier.scopusid | 2-s2.0-85059635480 | - |
dc.identifier.wosid | 000461396500011 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.24, no.1, pp.95 - 102 | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 24 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 95 | - |
dc.citation.endPage | 102 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002466882 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | NADH BINDING-SITE | - |
dc.subject.keywordPlus | CITRATE SYNTHASE | - |
dc.subject.keywordPlus | TRANSCRIPTIONAL REGULATION | - |
dc.subject.keywordPlus | TCA CYCLE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GLYCEROL | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | CRISPR | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | FNR | - |
dc.subject.keywordAuthor | 1,3-propanediol | - |
dc.subject.keywordAuthor | glycerol | - |
dc.subject.keywordAuthor | citrate synthase | - |
dc.subject.keywordAuthor | ArcA | - |
dc.subject.keywordAuthor | Klebsiella pneumoniae | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.