A new coccolith modified electrode-based biosensor using a cognate pair of aptamers with sandwich-type binding
DC Field | Value | Language |
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dc.contributor.author | Kim, Sang Hoon | - |
dc.contributor.author | Nam, Onyou | - |
dc.contributor.author | Jin, EonSeon | - |
dc.contributor.author | Gu, Man Bock | - |
dc.date.accessioned | 2021-09-01T21:43:01Z | - |
dc.date.available | 2021-09-01T21:43:01Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-01-01 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/68368 | - |
dc.description.abstract | In this study, we report a cognate pair of the aptamer-based sandwich-type electrochemical biosensor for type 2 diabetes biomarker (Vaspin) using coccolith modified electrodeposited on the screen-printed gold electrode (CME-SPGE). The coccolith derived from E. huxleyi used in this study were known to be highly-structured microparticles with many nano-sized pores. The CME-SPGE was successfully fabricated by drop-casting coccoliths, followed by Au sputtering and electrodeposition of Au. On this CME-SPGE electrode, the sandwich-type electrochemical aptasensor was fabricated by using a cognate pair of aptamers. The morphological, electrochemical characteristics and the performances of both the CME-SPGE and the completely fabricated sandwich type aptasensor were investigated by SEM, EDAX, cyclic voltammetry, and chronoamperometry. Due to the synergic effect of a cognate pair of aptamers on CME-SPGE, this newly developed sandwich-type electrochemical biosensor for Vaspin showed high specificity, and good sensitivity with a limit of detection (LOD) of 298 pM, along with more widen the linear range. To the best of our knowledge, this is the first report about the use of a coccolith modified electrode with a cognate pair aptamer resulting in sandwich-type binding in an electrochemical biosensor. With the advantages of using highly-structured biomineral microparticles and a cognate pair of aptamers, this new study may pave the innovative way to design a novel sandwich-type electrochemical aptasensor platform. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject | SENSITIVE DETECTION | - |
dc.subject | GROWTH-FACTOR | - |
dc.subject | GOLD NANOPARTICLES | - |
dc.subject | SURFACE-AREA | - |
dc.subject | VASPIN | - |
dc.subject | AMPLIFICATION | - |
dc.subject | OBESITY | - |
dc.subject | CARBON | - |
dc.title | A new coccolith modified electrode-based biosensor using a cognate pair of aptamers with sandwich-type binding | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Gu, Man Bock | - |
dc.identifier.doi | 10.1016/j.bios.2018.08.021 | - |
dc.identifier.scopusid | 2-s2.0-85051699405 | - |
dc.identifier.wosid | 000450376600020 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.123, pp.160 - 166 | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 123 | - |
dc.citation.startPage | 160 | - |
dc.citation.endPage | 166 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | SENSITIVE DETECTION | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | GOLD NANOPARTICLES | - |
dc.subject.keywordPlus | SURFACE-AREA | - |
dc.subject.keywordPlus | VASPIN | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | OBESITY | - |
dc.subject.keywordPlus | CARBON | - |
dc.subject.keywordAuthor | A cognate pair of aptamers | - |
dc.subject.keywordAuthor | Sandwich-type binding | - |
dc.subject.keywordAuthor | Electrochemical biosensor | - |
dc.subject.keywordAuthor | Coccolith | - |
dc.subject.keywordAuthor | Chronoamperometry | - |
dc.subject.keywordAuthor | Type 2 diabetes | - |
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