Targeted knockout of phospholipase A(2) to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production
DC Field | Value | Language |
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dc.contributor.author | Shin, Ye Sol | - |
dc.contributor.author | Jeong, Jooyeon | - |
dc.contributor.author | Thu Ha Thi Nguyen | - |
dc.contributor.author | Kim, Jaoon Young Hwan | - |
dc.contributor.author | Jin, EonSeon | - |
dc.contributor.author | Sim, Sang Jun | - |
dc.date.accessioned | 2021-09-01T21:44:56Z | - |
dc.date.available | 2021-09-01T21:44:56Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-01 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/68384 | - |
dc.description.abstract | Biofuel derived from microalgae have several advantages over other oleaginous crops, however, still needs to be improved with its cost aspect and can be achieved by developing of a strain with improved lipid productivity. In this study, the CRISPR-Cas9 system was incorporated to carry out a target-specific knockout of the phospholipase A(2) gene in Chlamydomonas reinhardtii. The targeted gene encodes a key enzyme in the Lands cycle. As a result, the mutants showed a characteristic of increased diacylglycerol pool, followed by a higher accumulation of triacylglycerol without being significantly compensated with the cell growth. As a result, the overall lipid productivities of phospholipase A(2) knockout mutants have increased by up to 64.25% (to 80.92 g L-1 d(-1)). This study can provide crucial information for the biodiesel industry. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | LIFE-CYCLE ASSESSMENT | - |
dc.subject | TECHNOECONOMIC ANALYSIS | - |
dc.subject | TRIACYLGLYCEROL ACCUMULATION | - |
dc.subject | MICROALGAL BIOFUELS | - |
dc.subject | NITROGEN STARVATION | - |
dc.subject | NUCLEAR TRANSGENES | - |
dc.subject | EXPRESSION | - |
dc.subject | ALGAE | - |
dc.subject | PHOTOBIOREACTOR | - |
dc.subject | STRATEGIES | - |
dc.title | Targeted knockout of phospholipase A(2) to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sim, Sang Jun | - |
dc.identifier.doi | 10.1016/j.biortech.2018.09.121 | - |
dc.identifier.scopusid | 2-s2.0-85054180320 | - |
dc.identifier.wosid | 000448161200047 | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.271, pp.368 - 374 | - |
dc.relation.isPartOf | BIORESOURCE TECHNOLOGY | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 271 | - |
dc.citation.startPage | 368 | - |
dc.citation.endPage | 374 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Energy & Fuels | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Energy & Fuels | - |
dc.subject.keywordPlus | LIFE-CYCLE ASSESSMENT | - |
dc.subject.keywordPlus | TECHNOECONOMIC ANALYSIS | - |
dc.subject.keywordPlus | TRIACYLGLYCEROL ACCUMULATION | - |
dc.subject.keywordPlus | MICROALGAL BIOFUELS | - |
dc.subject.keywordPlus | NITROGEN STARVATION | - |
dc.subject.keywordPlus | NUCLEAR TRANSGENES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ALGAE | - |
dc.subject.keywordPlus | PHOTOBIOREACTOR | - |
dc.subject.keywordPlus | STRATEGIES | - |
dc.subject.keywordAuthor | Chlamydomonas reinhardtii | - |
dc.subject.keywordAuthor | Biofuel | - |
dc.subject.keywordAuthor | Triacylglycerol | - |
dc.subject.keywordAuthor | Phospholipase | - |
dc.subject.keywordAuthor | CRISPR-CAS9 | - |
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