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Experimental Applications of in situ Liver Perfusion Machinery for the Study of Liver Disease

Authors
Choi, Won-MookEun, Hyuk SooLee, Young-SunKim, Sun JunKim, Myung-HoLee, Jun-HeeShim, Young-RiKim, Hee-HoonKim, Ye EunYi, Hyon-SeungJeong, Won-Il
Issue Date
1월-2019
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Keywords
hemodynamics; immune cell; in situ perfusion; liver disease; metabolism
Citation
MOLECULES AND CELLS, v.42, no.1, pp.45 - 55
Indexed
SCIE
SCOPUS
KCI
Journal Title
MOLECULES AND CELLS
Volume
42
Number
1
Start Page
45
End Page
55
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/68469
DOI
10.14348/molcells.2018.0330
ISSN
1016-8478
Abstract
The liver is involved in a wide range of activities in vertebrates and some other animals, including metabolism, protein synthesis, detoxification, and the immune system. Until now, various methods have been devised to study liver diseases; however, each method has its own limitations. In situ liver perfusion machinery, originally developed in rats, has been successfully adapted to mice, enabling the study of liver diseases. Here we describe the protocol, which is a simple but widely applicable method for investigating the liver diseases. The liver is perfused in situ by cannulation of the portal vein and suprahepatic inferior vena cava (IVC), with antegrade closed circuit circulation completed by clamping the infrahepatic IVC. In situ liver perfusion can be utilized to evaluate immune cell migration and function, hemodynamics and related cellular reactions in each type of hepatic cells, and the metabolism of toxic or other compounds by changing the composition of the circulating media. In situ liver perfusion method maintains liver function and cell viability for up to 2 h. This study also describes an optional protocol using densitygradient centrifugation for the separation of different types of hepatic cells, allowing the determination of changes in each cell type. In summary, this method of in situ liver perfusion will be useful for studying liver diseases as a complement to other established methods.
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