Double amplified colorimetric detection of DNA using gold nanoparticles, enzymes and a catalytic hairpin assembly
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Chanho | - |
dc.contributor.author | Park, Hyunjun | - |
dc.contributor.author | Lee, Hye Jin | - |
dc.contributor.author | Lee, Hye Sun | - |
dc.contributor.author | Park, Kyong Hwa | - |
dc.contributor.author | Choi, Chang-Hwan | - |
dc.contributor.author | Na, Sungsoo | - |
dc.date.accessioned | 2021-09-01T21:59:45Z | - |
dc.date.available | 2021-09-01T21:59:45Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2019-01 | - |
dc.identifier.issn | 0026-3672 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/68486 | - |
dc.description.abstract | The authors describe an isothermal and ultrasensitive colorimetric DNA assay that consists of two amplification stages using enzymes and acatalytic hairpin assembly (CHA). The first stepconsists in the selective amplification of DNA using Klenow fragment and nicking enzyme. The second stepconsists in the amplification of the optical signal by using a catalytic hairpin assembly. After two amplification steps, the DNA reaction induces the aggregation of the red gold nanoparticles to give a blue color shift. The degree of aggregation can be quantified by measurement of the ratio of the UV-vis absorbances of the solutions at 620 and 524nm which are the wavelengths of the aggregated gold nanoparticles and bare gold nanoparticles. The detection limit is as low as 3.1 fM. Due to the use of a specific enzyme, only the desired DNAs will be detected. The method can be applied to the determination of DNA of various lengths. Despite the presence of large amounts of wildtype DNA, it can readily detect a target DNA. Conceivably, the technique has a large potential because of its high sensitivity and selectivity. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER WIEN | - |
dc.subject | SENSITIVE DETECTION | - |
dc.subject | EGFR MUTATION | - |
dc.subject | AMPLIFICATION | - |
dc.subject | POLYNUCLEOTIDES | - |
dc.subject | CLEAVAGE | - |
dc.subject | BIOPSY | - |
dc.subject | SENSOR | - |
dc.subject | ASSAY | - |
dc.subject | KRAS | - |
dc.subject | PCR | - |
dc.title | Double amplified colorimetric detection of DNA using gold nanoparticles, enzymes and a catalytic hairpin assembly | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Kyong Hwa | - |
dc.contributor.affiliatedAuthor | Na, Sungsoo | - |
dc.identifier.doi | 10.1007/s00604-018-3154-2 | - |
dc.identifier.scopusid | 2-s2.0-85058818293 | - |
dc.identifier.wosid | 000453796800010 | - |
dc.identifier.bibliographicCitation | MICROCHIMICA ACTA, v.186, no.1 | - |
dc.relation.isPartOf | MICROCHIMICA ACTA | - |
dc.citation.title | MICROCHIMICA ACTA | - |
dc.citation.volume | 186 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | SENSITIVE DETECTION | - |
dc.subject.keywordPlus | EGFR MUTATION | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | POLYNUCLEOTIDES | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | BIOPSY | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | KRAS | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordAuthor | Klenow fragment | - |
dc.subject.keywordAuthor | DNA sensor | - |
dc.subject.keywordAuthor | AuNPs | - |
dc.subject.keywordAuthor | UV-vis absorbance | - |
dc.subject.keywordAuthor | CHA | - |
dc.subject.keywordAuthor | Hairpin DNA | - |
dc.subject.keywordAuthor | Liquid biopsy | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.