Growth of candida albicans biofilm is inhibited by salvia miltiorrhiza [단삼에 의한 Candida albicans 바이오필름 발달의 억제]
- Authors
- Lee, H.-S.; Kim, Y.
- Issue Date
- 2019
- Publisher
- Korean Society for Microbiology and Biotechnology
- Keywords
- Antifungal; Biofilm; Candida albicans; Hypha-specific gene; Salvia miltiorrhiza
- Citation
- Microbiology and Biotechnology Letters, v.47, no.3, pp.465 - 472
- Indexed
- SCOPUS
KCI
- Journal Title
- Microbiology and Biotechnology Letters
- Volume
- 47
- Number
- 3
- Start Page
- 465
- End Page
- 472
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/70771
- DOI
- 10.4014/mbl.1901.01007
- ISSN
- 1598-642X
- Abstract
- Candida albicans is an opportunistic human pathogen that causes infections. Candidiasis is often related to antifungal resistance because the pathogen has the ability to form biofilms. In a previous study, we found that the Salvia miltiorriza ethanol extract demonstrated anticandidal activity by altering membrane permeability and inhibiting the cell wall synthesis in C. albicans. Our results here demonstrate that 78 µg/ ml of the S. miltiorriza extract significantly diminished the early stage biofilms formed by 10 clinical C. albicans isolates by 51.3%; this was analyzed by 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT) reduction assay. The effect of the S. miltiorrhiza extract on the adhesion of C. albicans cells to polystyrene plates and germ tube formation was examined via microscopic investigation. Although the density of the adhered cells was remarkably reduced up on incubation with 39 µg/ml S. miltiorrhiza extract, germ tube formation by C. albicans was rarely affected. Quantitative real-time PCR analysis showed that the S. miltiorrhiza extract downregulated the expression of C. albicans hypha-specific genes, EAP1 by 34.7% (p < 0.001), ALS1 by 45.0% (p < 0.001), ALS3 by 48.1% (p < 0.001), and ECE1 by 21.3% (p = 0.006), respectively. Our data suggest that the S. miltiorrhiza ethanol extract significantly inhibited the early stage of biofilm formation by C. albicans by interfering with cell adhesion, by downregulating EAP1, ALS1 and ALS3, and presumably by modifying the cell wall and membrane structure. © 2019, The Korean Society for Microbiology and Biotechnology
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biotechnology and Bioinformatics > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.