Development of quantum dot aptasensor and its portable analyzer for the detection of di-2-ethylhexyl phthalate
DC Field | Value | Language |
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dc.contributor.author | Lim, Hyun Jeong | - |
dc.contributor.author | Kim, A-Ru | - |
dc.contributor.author | Yoon, Moon-Young | - |
dc.contributor.author | You, Youngmin | - |
dc.contributor.author | Chua, Beelee | - |
dc.contributor.author | Son, Ahjeong | - |
dc.date.accessioned | 2021-09-02T02:09:37Z | - |
dc.date.available | 2021-09-02T02:09:37Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-12-15 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/71202 | - |
dc.description.abstract | We have developed a quantum dot aptasensor (QD-aptasensor) and its accompanying portable analyzer for the detection of di-2-ethylhexyl phthalate (DEHP). This sensor is based on a newly screened aptamer (60-mer) via SELEX and shows a binding affinity of 213 nmol/L with DEHP. The 60-mer aptamer together with its three shorter truncated aptamers (45, 28, and 22-mer) as well as three different DNA probes (12, 9, and 13-mer) were further investigated to form the best combination for the QD-aptasensor. Using a 22-mer-truncated aptamer and a 12-mer DNA probe combination, the QD-aptasensor demonstrated excellent DEHP sensitivity with an LOQ = 0.5 pg/mL as well as good selectivity in the presence of other phthalate analogs. The binding between the truncated aptamers and DEHP was also characterized. Finally, a QD-aptasensor-based portable analyzer was also developed, and its equivalence to the laboratory protocol was established with a correlation coefficient r = 0.86 for DEHP concentrations ranging from 0.0005 to 100 ng/mL. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject | TANDEM MASS-SPECTROMETRY | - |
dc.subject | BISPHENOL-A | - |
dc.subject | FLUORESCENCE IMMUNOASSAY | - |
dc.subject | LIQUID-CHROMATOGRAPHY | - |
dc.subject | GC-MS | - |
dc.subject | WATER | - |
dc.subject | DNA | - |
dc.subject | APTAMERS | - |
dc.subject | QUANTIFICATION | - |
dc.subject | CONTAMINATION | - |
dc.title | Development of quantum dot aptasensor and its portable analyzer for the detection of di-2-ethylhexyl phthalate | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chua, Beelee | - |
dc.identifier.doi | 10.1016/j.bios.2018.08.065 | - |
dc.identifier.scopusid | 2-s2.0-85052888818 | - |
dc.identifier.wosid | 000447819700001 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.121, pp.1 - 9 | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 121 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 9 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | TANDEM MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | BISPHENOL-A | - |
dc.subject.keywordPlus | FLUORESCENCE IMMUNOASSAY | - |
dc.subject.keywordPlus | LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | GC-MS | - |
dc.subject.keywordPlus | WATER | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | APTAMERS | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | CONTAMINATION | - |
dc.subject.keywordAuthor | Quantum dot aptasensor | - |
dc.subject.keywordAuthor | Di-2-ethylhexyl phthalate | - |
dc.subject.keywordAuthor | Aptamer | - |
dc.subject.keywordAuthor | Portable analyzer | - |
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