Accurate Quantification of N-Glycolylneuraminic Acid in Therapeutic Proteins Using Supramolecular Mass Spectrometry
DC Field | Value | Language |
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dc.contributor.author | Lee, Hyun Hee L. | - |
dc.contributor.author | Heo, Chae Eun | - |
dc.contributor.author | Seo, Nari | - |
dc.contributor.author | Yung, Seung Gyu | - |
dc.contributor.author | An, Hyun Joo | - |
dc.contributor.author | Kim, Hugh, I | - |
dc.date.accessioned | 2021-09-02T02:14:31Z | - |
dc.date.available | 2021-09-02T02:14:31Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-12-05 | - |
dc.identifier.issn | 0002-7863 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/71241 | - |
dc.description.abstract | Practical applications of innovative host-guest systems are challenging because of unexpected guest competitors and/or subtle environmental differences. Herein, a supramolecular mass spectrometry (MS)-based method using a synthetic host, cucurbit[7]uril (CB[7]), was developed for identifying and quantifying N-glycolylneuraminic acid (Neu5Gc) in therapeutic glycoproteins, which critically reduces drug efficacy. The development of a reliable derivatization-free analytical method for Neu5Gc is highly challenging because of the interference by the abundant N-acetylneuraminic acid (Neu5Ac). CB[7] recognized the subtle structural differences between Neu5Gc and Neu5Ac. Distinct host-guest interactions between CB[7] and the two sialic acids produced a highly linear relationship between the complexation and concentration proportions of the two sialic acids in MS. Furthermore, the developed method had sub-picomolar quantification limits and a wide range of applicability for diverse glycoproteins, demonstrating the potential utility of this method as a reliable assay of Neu5Gc in therapeutic glycoproteins. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | ANION-EXCHANGE CHROMATOGRAPHY | - |
dc.subject | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject | SIALIC ACIDS | - |
dc.subject | PHASE | - |
dc.subject | MICRODETERMINATION | - |
dc.subject | GLYCOPROTEINS | - |
dc.subject | PROTONATION | - |
dc.subject | ALGORITHMS | - |
dc.subject | CHEMISTRY | - |
dc.subject | BINDING | - |
dc.title | Accurate Quantification of N-Glycolylneuraminic Acid in Therapeutic Proteins Using Supramolecular Mass Spectrometry | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yung, Seung Gyu | - |
dc.contributor.affiliatedAuthor | Kim, Hugh, I | - |
dc.identifier.doi | 10.1021/jacs.8b07864 | - |
dc.identifier.scopusid | 2-s2.0-85053674262 | - |
dc.identifier.wosid | 000452693800024 | - |
dc.identifier.bibliographicCitation | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.140, no.48, pp.16528 - 16534 | - |
dc.relation.isPartOf | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | - |
dc.citation.title | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | - |
dc.citation.volume | 140 | - |
dc.citation.number | 48 | - |
dc.citation.startPage | 16528 | - |
dc.citation.endPage | 16534 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | ANION-EXCHANGE CHROMATOGRAPHY | - |
dc.subject.keywordPlus | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | SIALIC ACIDS | - |
dc.subject.keywordPlus | PHASE | - |
dc.subject.keywordPlus | MICRODETERMINATION | - |
dc.subject.keywordPlus | GLYCOPROTEINS | - |
dc.subject.keywordPlus | PROTONATION | - |
dc.subject.keywordPlus | ALGORITHMS | - |
dc.subject.keywordPlus | CHEMISTRY | - |
dc.subject.keywordPlus | BINDING | - |
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