Galectin-1 from conditioned medium of three-dimensional culture of adipose-derived stem cells accelerates migration and proliferation of human keratinocytes and fibroblasts
DC Field | Value | Language |
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dc.contributor.author | Kim, Min Ho | - |
dc.contributor.author | Wu, Wen Hao | - |
dc.contributor.author | Choi, Jee Hyun | - |
dc.contributor.author | Kim, Jihyun | - |
dc.contributor.author | Jun, Jin Hyun | - |
dc.contributor.author | Ko, Yong | - |
dc.contributor.author | Lee, Jong Hun | - |
dc.date.accessioned | 2021-09-02T02:20:27Z | - |
dc.date.available | 2021-09-02T02:20:27Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-12 | - |
dc.identifier.issn | 1067-1927 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/71277 | - |
dc.description.abstract | Keratinocytes and fibroblasts cells play important roles in the skin-wound healing process and are the cell types activated by trauma. Activated cells participate in epithelialization, granulation, scar tissue formation, wound remodeling, and angiogenesis via a series of cellular activities including migration and proliferation. Previous studies reported that the conditioned medium (CM) of adipose-derived stem cells (ADSCs) stimulated the migration and proliferation of cell types involved in the skin wound healing process; however, these studies only show ADSC-CM effects that were obtained using 2-dimensional (2D) culture. Recently, 3-dimensional (3D) culture has been considered as a more physiologically appropriate system than 2D culture for ADSC cultures; therefore, ADSC-CM was collected from 3D culture (ADSC-CM-3D) and compared with ADSC-CM from 2D culture (ADSC-CM-2D) to investigate the effects on the migration and proliferation of human keratinocytes (HaCaTs) and fibroblasts. The migrations of the HaCaT cells and fibroblasts were significantly higher with ADSC-CM-3D compared with the 2D culture; similarly, the proliferation of HaCaT cells was also highly stimulated by ADSC-CM-3D. Proteomic analyses of the ADSC-CM revealed that collagens and actins were highly expressed in the 3D-culture system. Chitinase 3-like 1 (CHI3L1), tissue inhibitor of metalloproteinases (TIMP), and galectin-1 were specifically expressed only in ADSC-CM-3D. Especially, through antibody neutralization, galectin-1 in ADSC-CM-3D was found to be an important factor for the migration of human keratinocytes. Therefore, these results suggest that ADSC-CM-3D was more effective in the wound healing than ADSC-CM-2D, and galectin-1 in ADSC-CM-3D was could be a promising option for skin-wound healing. Furthermore, the differential expressions of several ADSC-CM proteins between the 2D- and 3D-culture systems may be used as basic information for the development of efficient wound-healing strategies. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | BREAST REGRESSION PROTEIN-39 | - |
dc.subject | HUMAN DERMAL FIBROBLASTS | - |
dc.subject | CHITINASE-LIKE PROTEINS | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | INFLAMMATION | - |
dc.subject | CARCINOMA | - |
dc.subject | PATHWAYS | - |
dc.subject | TARGET | - |
dc.subject | CANCER | - |
dc.subject | OXYGEN | - |
dc.title | Galectin-1 from conditioned medium of three-dimensional culture of adipose-derived stem cells accelerates migration and proliferation of human keratinocytes and fibroblasts | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ko, Yong | - |
dc.identifier.doi | 10.1111/wrr.12579 | - |
dc.identifier.scopusid | 2-s2.0-85056803722 | - |
dc.identifier.wosid | 000450679300002 | - |
dc.identifier.bibliographicCitation | WOUND REPAIR AND REGENERATION, v.26, pp.S9 - S18 | - |
dc.relation.isPartOf | WOUND REPAIR AND REGENERATION | - |
dc.citation.title | WOUND REPAIR AND REGENERATION | - |
dc.citation.volume | 26 | - |
dc.citation.startPage | S9 | - |
dc.citation.endPage | S18 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Dermatology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Dermatology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.subject.keywordPlus | BREAST REGRESSION PROTEIN-39 | - |
dc.subject.keywordPlus | HUMAN DERMAL FIBROBLASTS | - |
dc.subject.keywordPlus | CHITINASE-LIKE PROTEINS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | CARCINOMA | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | TARGET | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | OXYGEN | - |
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