Development and Evaluation of Hyaluronic Acid-Based Hybrid Bio-Ink for Tissue Regeneration
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jaeyeon | - |
dc.contributor.author | Lee, Se-Hwan | - |
dc.contributor.author | Kim, Byung Soo | - |
dc.contributor.author | Cho, Young-Sam | - |
dc.contributor.author | Park, Yongdoo | - |
dc.date.accessioned | 2021-09-02T02:21:35Z | - |
dc.date.available | 2021-09-02T02:21:35Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-12 | - |
dc.identifier.issn | 1738-2696 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/71286 | - |
dc.description.abstract | Bioprinting has recently appeared as a powerful tool for building complex tissue and organ structures. However, the application of bioprinting to regenerative medicine has limitations, due to the restricted choices of bio-ink for cytocompatible cell encapsulation and the integrity of the fabricated structures. In this study, we developed hybrid bio-inks based on acrylated hyaluronic acid (HA) for immobilizing bio-active peptides and tyramine-conjugated hyaluronic acids for fast gelation. Conventional acrylated HA-based hydrogels have a gelation time of more than 30 min, whereas hybrid bio-ink has been rapidly gelated within 200 s. Fibroblast cells cultured in this hybrid bio-ink up to 7 days showed > 90% viability. As a guidance cue for stem cell differentiation, we immobilized four different bio-active peptides: BMP-7-derived peptides (BMP-7D) and osteopontin for osteogenesis, and substance-P (SP) and Ac-SDKP (SDKP) for angiogenesis. Mesenchymal stem cells cultured in these hybrid bio-inks showed the highest angiogenic and osteogenic activity cultured in bio-ink immobilized with a SP or BMP-7D peptide. This bio-ink was loaded in a three-dimensional (3D) bioprinting device showing reproducible printing features. We have developed bio-inks that combine biochemical and mechanical cues. Biochemical cues were able to regulate differentiation of cells, and mechanical cues enabled printing structuring. This multi-functional bio-ink can be used for complex tissue engineering and regenerative medicine. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC | - |
dc.subject | SUBSTANCE-P | - |
dc.subject | STEM-CELLS | - |
dc.subject | OSTEOGENIC ACTIVITY | - |
dc.subject | VALVE CONDUITS | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | HYDROGELS | - |
dc.subject | OSTEOPONTIN | - |
dc.subject | PEPTIDE | - |
dc.subject | PROLIFERATION | - |
dc.subject | ANGIOGENESIS | - |
dc.title | Development and Evaluation of Hyaluronic Acid-Based Hybrid Bio-Ink for Tissue Regeneration | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Byung Soo | - |
dc.contributor.affiliatedAuthor | Park, Yongdoo | - |
dc.identifier.doi | 10.1007/s13770-018-0144-8 | - |
dc.identifier.scopusid | 2-s2.0-85056992784 | - |
dc.identifier.wosid | 000451373700007 | - |
dc.identifier.bibliographicCitation | TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.15, no.6, pp.761 - 769 | - |
dc.relation.isPartOf | TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.citation.title | TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.citation.volume | 15 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 761 | - |
dc.citation.endPage | 769 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002409684 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.subject.keywordPlus | SUBSTANCE-P | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | OSTEOGENIC ACTIVITY | - |
dc.subject.keywordPlus | VALVE CONDUITS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | HYDROGELS | - |
dc.subject.keywordPlus | OSTEOPONTIN | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordAuthor | Bio-ink | - |
dc.subject.keywordAuthor | Bioprinting | - |
dc.subject.keywordAuthor | Hyaluronic acid | - |
dc.subject.keywordAuthor | Hydrogel | - |
dc.subject.keywordAuthor | Tissue engineering | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.