Insights into Cell-Free Conversion of CO2 to Chemicals by a Multienzyme Cascade Reaction
DC Field | Value | Language |
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dc.contributor.author | Singh, Raushan Kumar | - |
dc.contributor.author | Singh, Ranjitha | - |
dc.contributor.author | Sivakumar, Dakshinamurthy | - |
dc.contributor.author | Kondaveeti, Sanath | - |
dc.contributor.author | Kim, Taedoo | - |
dc.contributor.author | Li, Jinglin | - |
dc.contributor.author | Sung, Bong Hyun | - |
dc.contributor.author | Cho, Byung-Kwan | - |
dc.contributor.author | Kim, Dong Rip | - |
dc.contributor.author | Kim, Sun Chang | - |
dc.contributor.author | Kalia, Vipin C. | - |
dc.contributor.author | Zhang, Yi-Heng P. Job | - |
dc.contributor.author | Zhao, Huimin | - |
dc.contributor.author | Kang, Yun Chan | - |
dc.contributor.author | Lee, Jung-Kul | - |
dc.date.accessioned | 2021-09-02T02:33:00Z | - |
dc.date.available | 2021-09-02T02:33:00Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-12 | - |
dc.identifier.issn | 2155-5435 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/71389 | - |
dc.description.abstract | Multienzymatic cascade reactions have garnered the attention of many researchers as an approach for converting CO, into methanol. The cascade reaction used in this study includes the following enzymes: a formate dehydrogenase (CIFDH), a formaldehyde dehydrogenase (BmFaldDH), and an alcohol dehydrogenase (YADH) from Clostridium ljungdahlii, Burkholderia multivorans, and Saccharomyces cerevisiae, respectively. Because this cascade reaction requires NADH as a cofactor, phosphite dehydrogenase (PTDH) was employed to regenerate the cofactor. The multienzymatic cascade reaction, along with PTDH, yielded 3.28 mM methanol. The key to the success of this cascade reaction was a novel formaldehyde dehydrogenase, BmFaldDH, the enzyme catalyzing the reduction of formate to formaldehyde. The methanol yield was further improved by incorporation of 1-ethyl-3-methylimidazolium acetate (EMIM-Ac), resulting in 7.86 mM of methanol. A 500-fold increase in total turnover number was observed for the CIFDH-BmFaldDH-YADH cascade system compared to the Candida boidinii FDH-Pseudomonas putida FaldDH-YADH system. We provided detailed insights into the enzymatic reduction of CO2 by determining the thermodynamic parameters (K-d and Delta G) using isothermal titration calorimetry. Furthermore, we demonstrated a novel time-dependent formaldehyde production from CO2. Our results will aid in the understanding and development of a robust multienzyme catalyzed cascade reaction for the reduction of CO2 to value-added chemicals. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | CARBON-DIOXIDE | - |
dc.subject | DIHYDROFOLATE-REDUCTASE | - |
dc.subject | ENZYMATIC CONVERSION | - |
dc.subject | METHANOL PRODUCTION | - |
dc.subject | DEHYDROGENASE | - |
dc.subject | CATALYSIS | - |
dc.subject | FORMATE | - |
dc.subject | ACID | - |
dc.title | Insights into Cell-Free Conversion of CO2 to Chemicals by a Multienzyme Cascade Reaction | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kang, Yun Chan | - |
dc.identifier.doi | 10.1021/acscatal.8b02646 | - |
dc.identifier.scopusid | 2-s2.0-85058292332 | - |
dc.identifier.wosid | 000453491100014 | - |
dc.identifier.bibliographicCitation | ACS CATALYSIS, v.8, no.12, pp.11085 - 11093 | - |
dc.relation.isPartOf | ACS CATALYSIS | - |
dc.citation.title | ACS CATALYSIS | - |
dc.citation.volume | 8 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 11085 | - |
dc.citation.endPage | 11093 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.subject.keywordPlus | CARBON-DIOXIDE | - |
dc.subject.keywordPlus | DIHYDROFOLATE-REDUCTASE | - |
dc.subject.keywordPlus | ENZYMATIC CONVERSION | - |
dc.subject.keywordPlus | METHANOL PRODUCTION | - |
dc.subject.keywordPlus | DEHYDROGENASE | - |
dc.subject.keywordPlus | CATALYSIS | - |
dc.subject.keywordPlus | FORMATE | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordAuthor | cascade reaction | - |
dc.subject.keywordAuthor | formaldehyde | - |
dc.subject.keywordAuthor | methanol | - |
dc.subject.keywordAuthor | multienzyme | - |
dc.subject.keywordAuthor | CO2 | - |
dc.subject.keywordAuthor | FDH | - |
dc.subject.keywordAuthor | FaldDH | - |
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