Novel Two-Step Process Utilizing a Single Enzyme for the Production of High-Titer 3,6-Anhydro-L-galactose from Agarose Derived from Red Macroalgae
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Dong Hyun | - |
dc.contributor.author | Yun, Eun Ju | - |
dc.contributor.author | Lee, Sang-Hyun | - |
dc.contributor.author | Kim, Kyoung Heon | - |
dc.date.accessioned | 2021-09-02T03:18:34Z | - |
dc.date.available | 2021-09-02T03:18:34Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-11-21 | - |
dc.identifier.issn | 0021-8561 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/71813 | - |
dc.description.abstract | 3,6-Anhydro-L-galactose (L-AHG), a major component of agarose derived from red macroalgae, has excellent potential for industrial applications based on its physiological activities such as skin whitening, moisturizing, anticariogenicity, and anti-inflammation. However, L-AHG is not yet commercially available due to the complexity, inefficiency, and high cost of the current processes for producing L-AHG. Currently, L-AHG production depends on a multistep process requiring several enzymes. Here, we designed and tested a novel two-step process for obtaining high-titer L-AHG by using a single enzyme. First, to depolymerize agarose preferentially into agarobiose (AB) at a high titer, the agarose prehydrolysis using phosphoric acid as a catalyst was optimized at a 30.7% (w/v) agarose loading, which is the highest agarose or agar loading reported so far. Then AB produced by the prehydrolysis was hydrolyzed into L-AHG and D-galactose (D-Gal) by using a recently discovered enzyme, BgI1B. We suggest that this simple and efficient process could be a feasible solution for the commercialization and mass production of L-AHG. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | GEL TRANSITION-TEMPERATURE | - |
dc.subject | ACID-HYDROLYSIS | - |
dc.subject | BETA-AGARASE | - |
dc.subject | SACCHARIFICATION | - |
dc.subject | PRETREATMENT | - |
dc.subject | BIOETHANOL | - |
dc.subject | MECHANISM | - |
dc.subject | RESOURCE | - |
dc.subject | ETHANOL | - |
dc.title | Novel Two-Step Process Utilizing a Single Enzyme for the Production of High-Titer 3,6-Anhydro-L-galactose from Agarose Derived from Red Macroalgae | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Dong Hyun | - |
dc.contributor.affiliatedAuthor | Yun, Eun Ju | - |
dc.contributor.affiliatedAuthor | Kim, Kyoung Heon | - |
dc.identifier.doi | 10.1021/acs.jafc.8b04144 | - |
dc.identifier.scopusid | 2-s2.0-85056803509 | - |
dc.identifier.wosid | 000451496600011 | - |
dc.identifier.bibliographicCitation | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.66, no.46, pp.12249 - 12256 | - |
dc.relation.isPartOf | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | - |
dc.citation.title | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | - |
dc.citation.volume | 66 | - |
dc.citation.number | 46 | - |
dc.citation.startPage | 12249 | - |
dc.citation.endPage | 12256 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | GEL TRANSITION-TEMPERATURE | - |
dc.subject.keywordPlus | ACID-HYDROLYSIS | - |
dc.subject.keywordPlus | BETA-AGARASE | - |
dc.subject.keywordPlus | SACCHARIFICATION | - |
dc.subject.keywordPlus | PRETREATMENT | - |
dc.subject.keywordPlus | BIOETHANOL | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | RESOURCE | - |
dc.subject.keywordPlus | ETHANOL | - |
dc.subject.keywordAuthor | 3,6-anhydro-L-galactose | - |
dc.subject.keywordAuthor | agarose | - |
dc.subject.keywordAuthor | red macroalgae | - |
dc.subject.keywordAuthor | agarobiose | - |
dc.subject.keywordAuthor | phosphoric acid | - |
dc.subject.keywordAuthor | agarobiose hydrolase | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.