Lysyl-Transfer RNA Synthetase Induces the Maturation of Dendritic Cells through MAPK and NF-kappa B Pathways, Strongly Contributing to Enhanced Th1 Cell Responses
DC Field | Value | Language |
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dc.contributor.author | Lim, Hui Xuan | - |
dc.contributor.author | Jung, Hak-Jun | - |
dc.contributor.author | Lee, Arim | - |
dc.contributor.author | Park, Si Hoon | - |
dc.contributor.author | Han, Byung Woo | - |
dc.contributor.author | Cho, Daeho | - |
dc.contributor.author | Kim, Tae Sung | - |
dc.date.accessioned | 2021-09-02T04:05:23Z | - |
dc.date.available | 2021-09-02T04:05:23Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-11-01 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/71900 | - |
dc.description.abstract | In addition to essential roles in protein synthesis, lysyl-tRNA synthetase (KRS) is secreted to trigger a proinflammatory function that induces macrophage activation and TNF-alpha secretion. KRS has been associated with autoimmune diseases such as polymyositis and dermatomyositis. In this study, we investigated the immunomodulatory effects of KRS on bone marrow-derived dendritic cells (DCs) of C57BL/6 mice and subsequent polarization of Th cells and analyzed the underlying mechanisms. KRS-treated DCs increased the expression of cell surface molecules and proinflammatory cytokines associated with DC maturation and activation. Especially, KRS treatment significantly increased production of IL-12, a Th1-polarizing cytokine, in DCs. KRS triggered the nuclear translocation of the NF-kappa B p65 subunit along with the degradation of I kappa B proteins and the phosphorylation of MAPKs in DCs. Additionally, JNK, p38, and ERK inhibitors markedly recovered the degradation of I kappa B proteins, suggesting the involvement of MAPKs as the upstream regulators of NF-kappa B in the KRS-induced DC maturation and activation. Importantly, KRS-treated DCs strongly increased the differentiation of Th1 cells when cocultured with CD4(+ )T cells. The addition of anti- IL-12-neutralizing Ab abolished the secretion of IFN-gamma in the coculture, indicating that KRS induces Th1 cell response via DC-derived IL-12. Moreover, KRS enhanced the OVA-specific Th1 cell polarization in vivo following the adoptive transfer of OVA-pulsed DCs. Taken together, these results indicated that KRS effectively induced the maturation and activation of DCs through MAPKs/NF-kappa B-signaling pathways and favored DC-mediated Th1 cell response. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER ASSOC IMMUNOLOGISTS | - |
dc.subject | LAMININ RECEPTOR | - |
dc.subject | IFN-GAMMA | - |
dc.subject | IN-VITRO | - |
dc.subject | CANCER | - |
dc.subject | POLARIZATION | - |
dc.subject | INFLAMMATION | - |
dc.subject | CYTOKINES | - |
dc.subject | MACROPHAGES | - |
dc.subject | PROGRESSION | - |
dc.subject | EXPRESSION | - |
dc.title | Lysyl-Transfer RNA Synthetase Induces the Maturation of Dendritic Cells through MAPK and NF-kappa B Pathways, Strongly Contributing to Enhanced Th1 Cell Responses | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cho, Daeho | - |
dc.contributor.affiliatedAuthor | Kim, Tae Sung | - |
dc.identifier.doi | 10.4049/jimmunol.1800386 | - |
dc.identifier.scopusid | 2-s2.0-85055198716 | - |
dc.identifier.wosid | 000447907700028 | - |
dc.identifier.bibliographicCitation | JOURNAL OF IMMUNOLOGY, v.201, no.9, pp.2832 - 2841 | - |
dc.relation.isPartOf | JOURNAL OF IMMUNOLOGY | - |
dc.citation.title | JOURNAL OF IMMUNOLOGY | - |
dc.citation.volume | 201 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 2832 | - |
dc.citation.endPage | 2841 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | LAMININ RECEPTOR | - |
dc.subject.keywordPlus | IFN-GAMMA | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | POLARIZATION | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | CYTOKINES | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordPlus | PROGRESSION | - |
dc.subject.keywordPlus | EXPRESSION | - |
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