Nucleotide triphosphatase and RNA chaperone activities of murine norovirus NS3
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Kang Rok | - |
dc.contributor.author | Lee, Ji-Hye | - |
dc.contributor.author | Kotiguda, Giri Gowda | - |
dc.contributor.author | Jung, Kyoung Ho | - |
dc.contributor.author | Chung, Mi Sook | - |
dc.contributor.author | Kang, Soowon | - |
dc.contributor.author | Hwang, Seungmin | - |
dc.contributor.author | Kim, Kyung Hyun | - |
dc.date.accessioned | 2021-09-02T04:23:51Z | - |
dc.date.available | 2021-09-02T04:23:51Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-11 | - |
dc.identifier.issn | 0022-1317 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/72058 | - |
dc.description.abstract | Modulation of RNA structure is essential in the life cycle of RNA viruses. Immediate replication upon infection requires RNA unwinding to ensure that RNA templates are not in intra-or intermolecular duplex forms. The calicivirus NS3, one of the highly conserved nonstructural (NS) proteins, has conserved motifs common to helicase superfamily 3 among six genogroups. However, its biological functions are not fully understood. In this study we report the oligomeric state and the nucleotide triphosphatase (NTPase) and RNA chaperone activities of the recombinant full-length NS3 derived from murine norovirus (MNV). The MNV NS3 has an Mg2+-dependent NTPase activity, and site-directed mutagenesis of the conserved NTPase motifs blocked enzyme activity and viral replication in cells. Further, the NS3 was found via fluorescence resonance energy transfer (FRET)-based assays to destabilize double-stranded RNA in the presence of Mg2+ or Mn2+ in an NTP-independent manner. However, the RNA destabilization activity was not affected by mutagenesis of the conserved motifs of NTPase. These results reveal that the MNV NS3 has an NTPase-independent RNA chaperone-like activity, and that a FRET-based RNA destabilization assay has the potential to identify new antiviral drugs targeting NS3. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MICROBIOLOGY SOC | - |
dc.subject | NUCLEIC-ACID-CHAPERONE | - |
dc.subject | NORWALK-LIKE VIRUS | - |
dc.subject | HEPATITIS-C VIRUS | - |
dc.subject | SUBGENOMIC PROMOTER | - |
dc.subject | ATPASE ACTIVITIES | - |
dc.subject | REPLICATION | - |
dc.subject | PROTEIN | - |
dc.subject | HELICASE | - |
dc.subject | COMPLEX | - |
dc.subject | CELLS | - |
dc.title | Nucleotide triphosphatase and RNA chaperone activities of murine norovirus NS3 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Ji-Hye | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hyun | - |
dc.identifier.doi | 10.1099/jgv.0.001151 | - |
dc.identifier.scopusid | 2-s2.0-85055773545 | - |
dc.identifier.wosid | 000448919800004 | - |
dc.identifier.bibliographicCitation | JOURNAL OF GENERAL VIROLOGY, v.99, no.11, pp.1482 - 1493 | - |
dc.relation.isPartOf | JOURNAL OF GENERAL VIROLOGY | - |
dc.citation.title | JOURNAL OF GENERAL VIROLOGY | - |
dc.citation.volume | 99 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1482 | - |
dc.citation.endPage | 1493 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Virology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Virology | - |
dc.subject.keywordPlus | NUCLEIC-ACID-CHAPERONE | - |
dc.subject.keywordPlus | NORWALK-LIKE VIRUS | - |
dc.subject.keywordPlus | HEPATITIS-C VIRUS | - |
dc.subject.keywordPlus | SUBGENOMIC PROMOTER | - |
dc.subject.keywordPlus | ATPASE ACTIVITIES | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | HELICASE | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | norovirus | - |
dc.subject.keywordAuthor | RNA replication | - |
dc.subject.keywordAuthor | NS3 | - |
dc.subject.keywordAuthor | NTPase | - |
dc.subject.keywordAuthor | helicase | - |
dc.subject.keywordAuthor | RNA chaperone | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.