3D-cultured human placenta-derived mesenchymal stem cell spheroids enhance ovary function by inducing folliculogenesis
DC Field | Value | Language |
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dc.contributor.author | Kim, Tae-Hee | - |
dc.contributor.author | Choi, Jong Ho | - |
dc.contributor.author | Jun, Yesl | - |
dc.contributor.author | Lim, Seung Mook | - |
dc.contributor.author | Park, Sohae | - |
dc.contributor.author | Paek, Jin-Young | - |
dc.contributor.author | Lee, Sang-Hoon | - |
dc.contributor.author | Hwang, Ji-Young | - |
dc.contributor.author | Kim, Gi Jin | - |
dc.date.accessioned | 2021-09-02T05:05:16Z | - |
dc.date.available | 2021-09-02T05:05:16Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-10-17 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/72467 | - |
dc.description.abstract | Placenta-derived mesenchymal stem cells (PD-MSCs) have numerous advantages over other adult MSCs that make them an attractive cell source for regenerative medicine. Here, we demonstrate the therapeutic effect of PD-MSCs in ovariectomized (Ovx) rats and compare their efficacy when generated via a conventional monolayer culture system (2D, naive) and a spheroid culture system (3D, spheroid). PD-MSC transplantation significantly increased the estradiol level in Ovx rats compared with the non-transplantation (NTx) group. In particular, the estradiol level in the Spheroid group was significantly higher than that in the Naive group at 2 weeks. Spheroid PD-MSCs exhibited a significantly higher efficiency of engraftment onto ovarian tissues at 2 weeks. The mRNA and protein expression levels of Nanos3, Nobox, and Lhx8 were also significantly increased in the Spheroid group compared with those in the NTx group at 1 and 2 weeks. These results suggest that PD-MSC transplantation can restore ovarian function in Ovx rats by increasing estrogen production and enhancing folliculogenesis-related gene expression levels and further indicate that spheroid-cultured PD-MSCs have enhanced therapeutic potential via increased engraftment efficiency. These findings improve our understanding of stem-cell-based therapies for reproductive systems and may suggest new avenues for developing efficient therapies using 3D cultivation systems. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | CONCAVE-MICROWELL ARRAYS | - |
dc.subject | BONE-MARROW | - |
dc.subject | IN-VITRO | - |
dc.subject | CORD BLOOD | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | MODEL | - |
dc.subject | PROLIFERATION | - |
dc.subject | MACROPHAGES | - |
dc.subject | PREMATURE | - |
dc.subject | INVASION | - |
dc.title | 3D-cultured human placenta-derived mesenchymal stem cell spheroids enhance ovary function by inducing folliculogenesis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, Ji-Young | - |
dc.identifier.doi | 10.1038/s41598-018-33575-9 | - |
dc.identifier.scopusid | 2-s2.0-85055080598 | - |
dc.identifier.wosid | 000447511100020 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.8 | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 8 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | CONCAVE-MICROWELL ARRAYS | - |
dc.subject.keywordPlus | BONE-MARROW | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | CORD BLOOD | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordPlus | PREMATURE | - |
dc.subject.keywordPlus | INVASION | - |
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