Stabilization of Bovine carbonic anhydrase II through rational site-specific immobilization
DC Field | Value | Language |
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dc.contributor.author | Lee, Chang Hyun | - |
dc.contributor.author | Jang, Eui Kyoung | - |
dc.contributor.author | Yeon, Young Joo | - |
dc.contributor.author | Pack, Seung Pil | - |
dc.date.accessioned | 2021-09-02T05:08:48Z | - |
dc.date.available | 2021-09-02T05:08:48Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-10-15 | - |
dc.identifier.issn | 1369-703X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/72477 | - |
dc.description.abstract | Carbonic anhydrase (CA) is a prominent biocatalyst used for the enzymatic CO2 capture process. For industrial applications, the immobilization of enzyme on a matrix support is a useful technique for stabilizing the enzyme and improving its reusability. Although there have been several trials for immobilization of CA, there is no systematic approach to investigate which site-specific immobilization is more effective for stabilization of CA. In this study, we investigated the effect of the immobilization position on the stability of CA using a-type Bovine CA (bCAII). Six candidate residues (K9, K36, T85, 0151, E233, or N252) were selected and each Cys mutant was site-specifically immobilized on the magnetic beads. The thermal and long-term stabilities were compared. Interestingly, the immobilized K9C and K36C, in which the immobilized sites are located close to the N-terminus of bCAll, showed 4.0- and 9.8-fold enhanced thermostability, respectively, at 58 degrees C. They also maintained 60.6% and 55.5% of activity, respectively, at 45 degrees C after 20 days, when the wild-type bCAII (free and randomly immobilized) completely lost its activity. These results indicated that the site-specific immobilization of the flexible residues on the N-terminal region could be an effective strategy for the stabilization of bCAll, which would be useful guidelines for the immobilization of other CAs in a site-specific manner. (C) 2018 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | CO2 SEQUESTRATION | - |
dc.subject | COVALENT IMMOBILIZATION | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | STABILITY | - |
dc.subject | EXPRESSION | - |
dc.subject | PROTEINS | - |
dc.subject | CAPTURE | - |
dc.subject | ENZYME | - |
dc.subject | FLEXIBILITY | - |
dc.subject | MACROMOLECULES | - |
dc.title | Stabilization of Bovine carbonic anhydrase II through rational site-specific immobilization | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Pack, Seung Pil | - |
dc.identifier.doi | 10.1016/j.bej.2018.06.019 | - |
dc.identifier.scopusid | 2-s2.0-85049437656 | - |
dc.identifier.wosid | 000445980900004 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL ENGINEERING JOURNAL, v.138, pp.29 - 36 | - |
dc.relation.isPartOf | BIOCHEMICAL ENGINEERING JOURNAL | - |
dc.citation.title | BIOCHEMICAL ENGINEERING JOURNAL | - |
dc.citation.volume | 138 | - |
dc.citation.startPage | 29 | - |
dc.citation.endPage | 36 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | CO2 SEQUESTRATION | - |
dc.subject.keywordPlus | COVALENT IMMOBILIZATION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | CAPTURE | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | FLEXIBILITY | - |
dc.subject.keywordPlus | MACROMOLECULES | - |
dc.subject.keywordAuthor | Carbonic anhydrase | - |
dc.subject.keywordAuthor | Site-specific immobilization | - |
dc.subject.keywordAuthor | Enzyme stability | - |
dc.subject.keywordAuthor | Quasi-rigid domain analysis | - |
dc.subject.keywordAuthor | Enzyme flexibility | - |
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