Characterization of inhibitory constituents of NO production from Catalpa ovata using LC-MS coupled with a cell-based assay
DC Field | Value | Language |
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dc.contributor.author | Park, Sangmin | - |
dc.contributor.author | Shin, Hyeji | - |
dc.contributor.author | Park, Yeeun | - |
dc.contributor.author | Choi, Ilgyu | - |
dc.contributor.author | Park, Byoungduck | - |
dc.contributor.author | Lee, Ki Yong | - |
dc.date.accessioned | 2021-09-02T05:31:54Z | - |
dc.date.available | 2021-09-02T05:31:54Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-10 | - |
dc.identifier.issn | 0045-2068 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/72643 | - |
dc.description.abstract | An effective screening method for inhibitors of NO production in natural products using LC-QTOF MS/MS coupled with a cell-based assay was proposed. The ethyl acetate fraction of Catalpa ovata exhibited a strong inhibitory effect on NO production in lipopolysaccharide-induced BV2 microglia cells. We attempted to identify the active constituents of C. ovata by using LC-QTOF MS/MS coupled with a cell-based assay. Peaks at approximately 14-15 min on the MS chromatogram were estimated to be the bioactive constituents. A new iridoid compound, 6-O-trans-feruloyl-3 beta-hydroxy-7-deoxyrehamaglutin A (4), and nine known compounds (1-3, 5-10) were isolated from the ethyl acetate fraction of C. ovata by repeated column chromatography. Compounds 3, 4, 5, 7, and 8 significantly attenuated lipopolysaccharide-stimulated NO production in BV2 cells. Our results indicate that LC-QTOF MS/MS coupled with a cell-based NO production inhibitory assay successfully predicted active compounds without a time-consuming isolation process. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | NITRIC-OXIDE | - |
dc.subject | G.-DON | - |
dc.subject | PHENOLIC-COMPOUNDS | - |
dc.subject | FALLEN LEAVES | - |
dc.subject | GLYCOSIDES | - |
dc.subject | NAPHTHOQUINONES | - |
dc.subject | FLAVONOIDS | - |
dc.subject | SCAVENGERS | - |
dc.title | Characterization of inhibitory constituents of NO production from Catalpa ovata using LC-MS coupled with a cell-based assay | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Ki Yong | - |
dc.identifier.doi | 10.1016/j.bioorg.2018.05.023 | - |
dc.identifier.scopusid | 2-s2.0-85047834747 | - |
dc.identifier.wosid | 000441537800006 | - |
dc.identifier.bibliographicCitation | BIOORGANIC CHEMISTRY, v.80, pp.57 - 63 | - |
dc.relation.isPartOf | BIOORGANIC CHEMISTRY | - |
dc.citation.title | BIOORGANIC CHEMISTRY | - |
dc.citation.volume | 80 | - |
dc.citation.startPage | 57 | - |
dc.citation.endPage | 63 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Organic | - |
dc.subject.keywordPlus | GLYCOSIDES | - |
dc.subject.keywordPlus | NAPHTHOQUINONES | - |
dc.subject.keywordPlus | FLAVONOIDS | - |
dc.subject.keywordPlus | SCAVENGERS | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | G.-DON | - |
dc.subject.keywordPlus | PHENOLIC-COMPOUNDS | - |
dc.subject.keywordPlus | FALLEN LEAVES | - |
dc.subject.keywordAuthor | Catalpa ovata | - |
dc.subject.keywordAuthor | Bignoniaceae | - |
dc.subject.keywordAuthor | Chemical profile | - |
dc.subject.keywordAuthor | LC-QTOF MS/MS | - |
dc.subject.keywordAuthor | Anti-inflammatory | - |
dc.subject.keywordAuthor | NO production | - |
dc.subject.keywordAuthor | LPS | - |
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