Development of a Novel Nonradioisotopic Assay and Cdc25B Overexpression Cell Lines for Use in Screening for Cdc25B Inhibitors
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ha, Gyong Sik | - |
dc.contributor.author | Lee, Chung Min | - |
dc.contributor.author | Kim, Chan-Wha | - |
dc.date.accessioned | 2021-09-02T06:10:11Z | - |
dc.date.available | 2021-09-02T06:10:11Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-10 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/73024 | - |
dc.description.abstract | Purpose: The cyclin-dependent kinase 1 (Cdk1) and cyclin B complex performs important roles in the transition from the G2 to M phase in the cell cycle through removal of inhibitory phosphates on Cdk1, and Cdc25B, which is a dual-specific phosphatase, mediates these dephosphorylation events. However, measuring Cdc25B activity by existing methods is hampered by inadequate nonspecific substrates and the need to use a radiolabeled isotope. The present study aimed to develop an improved method with which to properly measure Cdc25B activity using a novel nonradioisotopic assay and Cdc25B overexpression cell lines. Materials and Methods: A nonradioisotopic Cdk1 kinase assay, based on Western blotting for retinoblastoma protein and histone H1, was used to analyze Cdc25B activity. Also, stable Cdc25B2 and Cdc25B3 overexpression HeLa cell lines were constructed using the tetracycline-regulated expression system and were applied as a tool for screening for inhibitors of Cdc25B. Results: The present study developed and optimized a nonradioisotopic assay method to properly measure Cdc25B activity. Furthermore, we constructed stable Cdc25B2 and Cdc25B3 overexpression HeLa cell lines for the establishment of a strong assay system with which to evaluate the specificity of Cdc25B inhibitors under conditions similar to the intracellular environment. These methods were confirmed as useful tools for measuring Cdc25B activity. Conclusion: The nonradioisotopic Cdk1 kinase assay and Cdc25B overexpression cell lines developed in this study can be conveniently used as tools for screening inhibitors of Cdc25B phosphatase as anticancer drugs. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | YONSEI UNIV COLL MEDICINE | - |
dc.subject | PHOSPHATASE | - |
dc.subject | ACTIVATION | - |
dc.subject | MECHANISM | - |
dc.subject | PROTEIN | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | PROGRESSION | - |
dc.subject | TRANSITION | - |
dc.subject | PROGNOSIS | - |
dc.subject | VIMENTIN | - |
dc.subject | KINASES | - |
dc.title | Development of a Novel Nonradioisotopic Assay and Cdc25B Overexpression Cell Lines for Use in Screening for Cdc25B Inhibitors | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Chan-Wha | - |
dc.identifier.doi | 10.3349/ymj.2018.59.8.995 | - |
dc.identifier.scopusid | 2-s2.0-85054049122 | - |
dc.identifier.wosid | 000443831900013 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, v.59, no.8, pp.995 - 1003 | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.citation.title | YONSEI MEDICAL JOURNAL | - |
dc.citation.volume | 59 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 995 | - |
dc.citation.endPage | 1003 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002385309 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | General & Internal Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, General & Internal | - |
dc.subject.keywordPlus | PHOSPHATASE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | PROGRESSION | - |
dc.subject.keywordPlus | TRANSITION | - |
dc.subject.keywordPlus | PROGNOSIS | - |
dc.subject.keywordPlus | VIMENTIN | - |
dc.subject.keywordPlus | KINASES | - |
dc.subject.keywordAuthor | Cdc25B | - |
dc.subject.keywordAuthor | Cdk1 | - |
dc.subject.keywordAuthor | nonradioisotopic assay | - |
dc.subject.keywordAuthor | Cdc25B overexpression cell lines | - |
dc.subject.keywordAuthor | anticancer drugs | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.