Inhibition of STAT3 in gastric cancer: role of pantoprazole as SHP-1 inducer
DC Field | Value | Language |
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dc.contributor.author | Koh, Jin Sung | - |
dc.contributor.author | Joo, Moon Kyung | - |
dc.contributor.author | Park, Jong-Jae | - |
dc.contributor.author | Yoo, Hyo Soon | - |
dc.contributor.author | Choi, Byung Il | - |
dc.contributor.author | Lee, Beom Jae | - |
dc.contributor.author | Chun, Hoon Jai | - |
dc.contributor.author | Lee, Sang Woo | - |
dc.date.accessioned | 2021-09-02T06:29:27Z | - |
dc.date.available | 2021-09-02T06:29:27Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-09-06 | - |
dc.identifier.issn | 2045-3701 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/73129 | - |
dc.description.abstract | Background: We investigated the inhibitory effect of pantoprazole on signal transducer and activator of transcription 3 (STAT3) activity and invasiveness of gastric adenocarcinoma cells, and the role of SH2-containing protein tyrosine phosphatase 1 (SHP-1) in mediating role. Methods: We used AGS and MKN-28 cells because of reduced SHP-1 and preserved p-STAT3 expression. Western blot, wound closure assay, Matrigel invasion assay and 3-D culture invasion assay were performed. Pharmacologic inhibitor of SHP-1 and siRNA were used for validation of the role of SHP-1. Results: We observed that pantoprazole at 40, 80, and 160 mu g/ml upregulated SHP-1 and downregulated p-STAT3 expression in a dose-dependent manner in AGS and MKN-28 cells. Furthermore, pantoprazole significantly downregulated mesenchymal markers (Snail1 and vimentin), upregulated epithelial marker (E-cadherin), and inhibited migration and invasion of AGS and MKN-28 cells. To validate the role of SHP-1 in inhibition of STAT3 activity by pantoprazole in gastric cancer cells, we performed pharmacologic inhibition (pervanadate) or knockdown of SHP-1 before pantoprazole treatment, which significantly attenuated the suppression of p-STAT3 and anti-migration and invasion effect by pantoprazole in AGS cells. In xenograft tumor model, tumor volume was significantly reduced by intraperitoneal injection of pantoprazole, with upregulation of SHP-1 and downregulation of p-STAT3, which were attenuated by concomitant injection of pervanadate. Conclusion: Our data suggest that the inhibitory effect of pantoprazole on cellular migration and invasion might be through inducing SHP-1 in gastric cancer cells. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.subject | PROTON PUMP INHIBITOR | - |
dc.subject | TYROSINE-PHOSPHATASE SHP-1 | - |
dc.subject | SIGNALING PATHWAY | - |
dc.subject | TARGETING STAT3 | - |
dc.subject | BREAST-CANCER | - |
dc.subject | CELLS | - |
dc.subject | PROLIFERATION | - |
dc.subject | INDUCTION | - |
dc.subject | CHEMOTHERAPY | - |
dc.subject | PROGRESSION | - |
dc.title | Inhibition of STAT3 in gastric cancer: role of pantoprazole as SHP-1 inducer | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Joo, Moon Kyung | - |
dc.contributor.affiliatedAuthor | Park, Jong-Jae | - |
dc.contributor.affiliatedAuthor | Lee, Beom Jae | - |
dc.contributor.affiliatedAuthor | Chun, Hoon Jai | - |
dc.contributor.affiliatedAuthor | Lee, Sang Woo | - |
dc.identifier.doi | 10.1186/s13578-018-0248-9 | - |
dc.identifier.scopusid | 2-s2.0-85052954241 | - |
dc.identifier.wosid | 000444516800001 | - |
dc.identifier.bibliographicCitation | CELL AND BIOSCIENCE, v.8 | - |
dc.relation.isPartOf | CELL AND BIOSCIENCE | - |
dc.citation.title | CELL AND BIOSCIENCE | - |
dc.citation.volume | 8 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | PROTON PUMP INHIBITOR | - |
dc.subject.keywordPlus | TYROSINE-PHOSPHATASE SHP-1 | - |
dc.subject.keywordPlus | SIGNALING PATHWAY | - |
dc.subject.keywordPlus | TARGETING STAT3 | - |
dc.subject.keywordPlus | BREAST-CANCER | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | CHEMOTHERAPY | - |
dc.subject.keywordPlus | PROGRESSION | - |
dc.subject.keywordAuthor | Pantoprazole | - |
dc.subject.keywordAuthor | Gastric cancer | - |
dc.subject.keywordAuthor | SH2-containing protein tyrosine phosphatase 1 | - |
dc.subject.keywordAuthor | Signal transducer and activator of transcription 3 | - |
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