Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Kunse | - |
dc.contributor.author | Ko, Hae Li | - |
dc.contributor.author | Lee, Eun-Young | - |
dc.contributor.author | Park, Hyo-Jung | - |
dc.contributor.author | Kim, Young Seok | - |
dc.contributor.author | Kim, Yeon-Sook | - |
dc.contributor.author | Cho, Nam-Hyuk | - |
dc.contributor.author | Park, Man-Seong | - |
dc.contributor.author | Lee, Sang-Myeong | - |
dc.contributor.author | Kim, Jihye | - |
dc.contributor.author | Kim, Hun | - |
dc.contributor.author | Seong, Baik Lin | - |
dc.contributor.author | Nam, Jae-Hwan | - |
dc.date.accessioned | 2021-09-02T06:41:57Z | - |
dc.date.available | 2021-09-02T06:41:57Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-09 | - |
dc.identifier.issn | 0385-5600 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/73232 | - |
dc.description.abstract | Middle East respiratory syndrome coronavirus (MERS-CoV) is a single-stranded RNA virus that causes severe respiratory disease in humans with a high fatality rate. Binding of the receptor binding domain (RBD) of the spike (S) glycoprotein to dipeptidyl peptidase 4 is the critical step in MERS-CoV infection of a host cell. No vaccines or clinically applicable treatments are currently available for MERS-CoV. Therefore, rapid diagnosis is important for improving patient outcomes through prompt treatment and protection against viral outbreaks. In this study, the aim was to establish two ELISA systems for detecting antigens and antibodies against MERS-CoV. Using a recombinant full-length S protein, an indirect ELISA was developed and found to detect MERS-CoV-specific antibodies in animal sera and sera of patient with MERS. Moreover, MAbs were induced with the recombinant S protein and RBD and used for sandwich ELISA to detect the MERS-CoV S protein. Neither ELISA system exhibited significant intra-assay or inter-assay variation, indicating good reproducibility. Moreover, the inter-day precision and sensitivity were adequate for use as a diagnostic kit. Thus, these ELISAs can be used clinically to diagnose MERS-CoV. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | RECEPTOR-BINDING DOMAIN | - |
dc.subject | MERS-COV INFECTION | - |
dc.subject | SPIKE PROTEIN | - |
dc.subject | NEUTRALIZING ANTIBODIES | - |
dc.subject | ASSAYS | - |
dc.subject | MICE | - |
dc.title | Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Man-Seong | - |
dc.identifier.doi | 10.1111/1348-0421.12643 | - |
dc.identifier.scopusid | 2-s2.0-85053623478 | - |
dc.identifier.wosid | 000445193100004 | - |
dc.identifier.bibliographicCitation | MICROBIOLOGY AND IMMUNOLOGY, v.62, no.9, pp.574 - 584 | - |
dc.relation.isPartOf | MICROBIOLOGY AND IMMUNOLOGY | - |
dc.citation.title | MICROBIOLOGY AND IMMUNOLOGY | - |
dc.citation.volume | 62 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 574 | - |
dc.citation.endPage | 584 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | RECEPTOR-BINDING DOMAIN | - |
dc.subject.keywordPlus | MERS-COV INFECTION | - |
dc.subject.keywordPlus | SPIKE PROTEIN | - |
dc.subject.keywordPlus | NEUTRALIZING ANTIBODIES | - |
dc.subject.keywordPlus | ASSAYS | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordAuthor | ELISA | - |
dc.subject.keywordAuthor | Middle East respiratory syndrome coronavirus | - |
dc.subject.keywordAuthor | receptor binding domain | - |
dc.subject.keywordAuthor | spike protein | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.