B7-H1-mediated immunosuppressive properties in human mesenchymal stem cells are mediated by STAT-1 and not PI3K/Akt signaling
DC Field | Value | Language |
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dc.contributor.author | Jang, In Keun | - |
dc.contributor.author | Jung, Hyun Joo | - |
dc.contributor.author | Noh, O. Kyu | - |
dc.contributor.author | Lee, Doo-Hoon | - |
dc.contributor.author | Lee, Kwang Chul | - |
dc.contributor.author | Park, Jun Eun | - |
dc.date.accessioned | 2021-09-02T08:33:09Z | - |
dc.date.available | 2021-09-02T08:33:09Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-08 | - |
dc.identifier.issn | 1791-2997 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/74198 | - |
dc.description.abstract | Mesenchymal stem cells (MSCs), derived from either bone marrow (BM) or Wharton's jelly (WJ), inhibit the proliferation of activated T cells, and interferon (IFN)- serves an important role in this process. This process is B7-homolog (H)1-dependent during cell contact inhibition. However, the signaling pathway involved in B7-H1 expression in MSCs remains largely undefined. The present study demonstrated activation of B7-H1 by engaging signal transducer and activator of transcription (STAT)-1 signaling in MSCs. Human BM- and WJ-MSCs were isolated and cultured. The immunosuppressive effect of BM- and WJ-MSCs on phytohemagglutinin (PHA)-induced T cell proliferation was compared using direct and indirect co-culture systems. B7-H1 expression on BM- and WJ-MSCs was detected by flow cytometry. Small interfering (si)RNA was used to knock down the expression of STAT-1. The inhibitory effect of MSCs on T lymphocytes was observed using PHA-induced T cell proliferation assays. IFN--induced B7-H1 expression on human BM- and WJ-MSCs increased in a time-dependent manner. Furthermore, the inhibitory effect of MSCs on T cell proliferation was be restored when an anti-B7-H1 monoclonal antibody was used. When STAT-1 signaling was inhibited by siRNA, B7-H1 expression on IFN--treated MSCs decreased and T cell proliferation was restored; however, the expression of B7-H1 did not alter upon treatment with a phosphatidylinositol-3-kinase (PI3K) inhibitor (LY294002). These results demonstrated that the IFN--induced immunosuppressive properties of B7-H1 in human BM- and WJ-MSCs were mediated by STAT-1 signaling, and not by PI3K/RAC- serine/threonine-protein kinase signaling. Understanding the intracellular mechanisms underlying IFN--induced expression of B7-H1 in MSCs may ultimately lead to an improved understanding of MSCs and provide insight into their use as cell therapy agents. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.subject | STROMAL CELLS | - |
dc.subject | EXPRESSION | - |
dc.subject | B7-H1 | - |
dc.subject | GAMMA | - |
dc.subject | IDO | - |
dc.subject | TRANSPLANTATION | - |
dc.subject | PROLIFERATION | - |
dc.subject | CARCINOMA | - |
dc.subject | THERAPY | - |
dc.subject | CANCER | - |
dc.title | B7-H1-mediated immunosuppressive properties in human mesenchymal stem cells are mediated by STAT-1 and not PI3K/Akt signaling | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Kwang Chul | - |
dc.identifier.doi | 10.3892/mmr.2018.9102 | - |
dc.identifier.scopusid | 2-s2.0-85049589022 | - |
dc.identifier.wosid | 000440583800075 | - |
dc.identifier.bibliographicCitation | MOLECULAR MEDICINE REPORTS, v.18, no.2, pp.1842 - 1848 | - |
dc.relation.isPartOf | MOLECULAR MEDICINE REPORTS | - |
dc.citation.title | MOLECULAR MEDICINE REPORTS | - |
dc.citation.volume | 18 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 1842 | - |
dc.citation.endPage | 1848 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | B7-H1 | - |
dc.subject.keywordPlus | GAMMA | - |
dc.subject.keywordPlus | IDO | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | CARCINOMA | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordAuthor | B7-homolog 1 | - |
dc.subject.keywordAuthor | signal transducer and activator of transcription 1 | - |
dc.subject.keywordAuthor | mesenchymal stem cells | - |
dc.subject.keywordAuthor | interferon- | - |
dc.subject.keywordAuthor | phosphatidylinositol-3-kinase | - |
dc.subject.keywordAuthor | RAC- serine | - |
dc.subject.keywordAuthor | threonine-protein kinase | - |
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