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Identification of antioxidant constituents of the aerial part of Plantago asiatica using LC-MS/MS coupled DPPH assay
- Authors
- Ahn, Jong Hoon; Jo, Yang Hee; Kim, Seon Beom; Turk, Ayman; Oh, Kyung-Eon; Hwang, Bang Yeon; Lee, Ki Yong; Lee, Mi Kyeong
- Issue Date
- 8월-2018
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Plantago asiatica; Plantaginaceae; phenylethanoid; LC-MS/MS online assay; Iridoid
- Citation
- PHYTOCHEMISTRY LETTERS, v.26, pp.20 - 24
- Indexed
- SCIE
SCOPUS
- Journal Title
- PHYTOCHEMISTRY LETTERS
- Volume
- 26
- Start Page
- 20
- End Page
- 24
- ISSN
- 1874-3900
- Abstract
- Screening of EtOAc soluble fraction of Plantago asiatica (Plantaginaceae) using HPLC-MS/MS coupled DPPH online assay suggested the presence of antioxidant compounds, which characterized as phenylethanoid glycosides. Twenty-nine compounds including three new compounds, plantalides A-C, a phenylethanoid (1), a phenolic compound (9) and an iridoid (10), were isolated from P. asiatica. Consistent with HPLC-MS/MS analysis, seven phenylethanoid glycosides (1-7) including a new phenylethanoid glycoside (1) showed antioxidant with ranging IC50 values of 22.9-88.5 mu M. These results suggested that phenylethanoid glycosides were contributed to the antioxidant activity of P. asiatica and HPLC-MS/MS coupled online assay is powerful tool for predicting active compounds with short time and limited amounts.
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