Model-Based Complete Enzymatic Production of 3,6-Anhydro-L-galactose from Red Algal Biomass
- Authors
- Pathiraja, Duleepa; Lee, Saeyoung; Choi, In-Geol
- Issue Date
- 4-7월-2018
- Publisher
- AMER CHEMICAL SOC
- Keywords
- red algal biomass; agar; 3,6-anhydro-L-galactose; agarase; enzymatic saccharification; agarolytic pathway; empirical model
- Citation
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.66, no.26, pp.6814 - 6821
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
- Volume
- 66
- Number
- 26
- Start Page
- 6814
- End Page
- 6821
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/74374
- DOI
- 10.1021/acs.jafc.8b01792
- ISSN
- 0021-8561
- Abstract
- 3,6-Anhydro-L-galactose (L-AHG) is a bioactive constituent of agar polysaccharides. To be used as a cosmetic or pharmaceutical ingredient, L-AHG is more favorably prepared by enzymatic saccharification of agar using a combination of agarolytic enzymes. Determining the optimum enzyme combination from the natural repertoire is a bottleneck for designing an efficient enzymatic-hydrolysis process. We consider all theoretical enzymatic-saccharification routes in the natural agarolytic pathway of a marine bacterium, Saccharophagus degradans 2-40. Among these routes, three representative routes were determined by removing redundant enzymatic reactions. We simulated each L-AHG production route with simple kinetic models and validated the reaction feasibility with an experimental procedure. The optimal enzyme mixture (with 67.3% maximum saccharification yield) was composed of endotype beta-agarase, exotype beta-agarase, agarooligosaccharolytic beta-galactosidase, and alpha-neoagarobiose hydrolase. This approach will reduce the time and effort needed for developing a coherent enzymatic process to produce L-AHG on a mass scale.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
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