ER stress attenuation by Aloe-derived polysaccharides in the protection of pancreatic beta-cells from free fatty acid-induced lipotoxicity
DC Field | Value | Language |
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dc.contributor.author | Kim, Kisoo | - |
dc.contributor.author | Chung, Min Hwa | - |
dc.contributor.author | Park, Soyoung | - |
dc.contributor.author | Cha, Jimin | - |
dc.contributor.author | Baek, Jin Hong | - |
dc.contributor.author | Lee, Shin-Young | - |
dc.contributor.author | Choi, Sang-Yun | - |
dc.date.accessioned | 2021-09-02T10:19:23Z | - |
dc.date.available | 2021-09-02T10:19:23Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-06-07 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/74949 | - |
dc.description.abstract | Insulin resistance, a pathophysiology of type 2 diabetes, is associated with obesity. Lipotoxicity in obesity leads to the dysfunction and death of pancreatic beta-cells and inadequate insulin production, thereby aggravating type 2 diabetes. The present study was conducted to determine the effect of Aloe vera polysaccharides (APs) as an anti-hyperglycemic agent and their mechanisms of action. Gel polysaccharides from Aloe extracts were separated using ultrafiltration devices with molecular weight-cutoff membranes, and the protective effect of APs on pancreatic beta-cells in response to free fatty acids (FFAs) was determined. Hamster pancreatic beta-cell line HIT-T15 was treated with palmitate and APs to analyze cellular responses. We observed a large number of apoptotic beta-cell death after treatment with high levels of palmitate, but this was efficiently prevented by the addition of APs in a dose-dependent manner. It was found that the anti-apoptotic properties of APs were largely due to the relief of endoplasmic reticulum (ER) stress signaling. APs were effective in interfering with the FFA-induced activation of the PERK and IRE1 pathways as well as ROS generation, thereby protecting pancreatic beta-cells from lipotoxicity. Although variation in the chain length of APs can influence the activity of FFA-mediated ER stress signaling in different ways, polysaccharide mixtures with molecular weights higher than 50 kDa showed greater antiapoptotic and antioxidant activity in beta-cells. After oral administration of APs, markedly lowering fasting blood glucose levels were observed in db/db mice, providing evidence of the potential of APs as an alternative insulin sensitizer. Therefore, it was concluded that APs have a protective effect against type 2 diabetes by modulating obesity-induced ER stress in pancreatic beta-cells. (C) 2018 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | ENDOPLASMIC-RETICULUM STRESS | - |
dc.subject | RESISTANCE | - |
dc.subject | APOPTOSIS | - |
dc.title | ER stress attenuation by Aloe-derived polysaccharides in the protection of pancreatic beta-cells from free fatty acid-induced lipotoxicity | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Sang-Yun | - |
dc.identifier.doi | 10.1016/j.bbrc.2018.04.162 | - |
dc.identifier.scopusid | 2-s2.0-85046172142 | - |
dc.identifier.wosid | 000433267800043 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.500, no.3, pp.797 - 803 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 500 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 797 | - |
dc.citation.endPage | 803 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM STRESS | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordAuthor | Free fatty acid | - |
dc.subject.keywordAuthor | Aloe vera | - |
dc.subject.keywordAuthor | Diabetes | - |
dc.subject.keywordAuthor | Beta cell | - |
dc.subject.keywordAuthor | ER stress | - |
dc.subject.keywordAuthor | Apoptosis | - |
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