BrainFilm, a novel technique for physical compression of 3D brain slices for efficient image acquisition and post-processing
DC Field | Value | Language |
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dc.contributor.author | Kim, Joo Yeon | - |
dc.contributor.author | Kim, Hyun Jung | - |
dc.contributor.author | Jang, Min Jee | - |
dc.contributor.author | Kim, June Hoan | - |
dc.contributor.author | Lee, Ju-Hyun | - |
dc.contributor.author | Lee, Eunsoo | - |
dc.contributor.author | Park, Kyerl | - |
dc.contributor.author | Kim, Hyuncheol | - |
dc.contributor.author | Lee, Jaedong | - |
dc.contributor.author | Kwag, Jeehyun | - |
dc.contributor.author | Kim, Namhee | - |
dc.contributor.author | Song, Mi-Ryoung | - |
dc.contributor.author | Kim, Hyun | - |
dc.contributor.author | Sun, Woong | - |
dc.date.accessioned | 2021-09-02T10:21:44Z | - |
dc.date.available | 2021-09-02T10:21:44Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-06-04 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/74966 | - |
dc.description.abstract | Tissue clearing enables us to observe thick tissue at a single cell resolution by reducing light scattering and refractive index matching. However, imaging of a large volume of tissue for 3D reconstruction requires a great deal of time, cost, and efforts. Few methods have been developed to transcend these limitations by mechanical compression or isotropic tissue shrinkage. Tissue shrinkage significantly lessens the imaging burden; however, there is an inevitable trade-off with image resolution. Here, we have developed the "BrainFilm" technique to compress cleared tissue at Z-axis by dehydration, without alteration of the XY-axis. The Z-axis compression was approximately 90%, and resulted in substantial reduction in image acquisition time and data size. The BrainFilm technique was successfully used to trace and characterize the morphology of thick biocytin-labelled neurons following electrophysiological recording and trace the GFP-labelled long nerve projections in irregular tissues such as the limb of mouse embryo. Thus, BrainFilm is a versatile tool that can be applied in diverse studies of 3D tissues in which spatial information of the Z-axis is dispensable. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | 3-DIMENSIONAL VISUALIZATION | - |
dc.subject | WHOLE-BODY | - |
dc.subject | TISSUE | - |
dc.subject | MICROSCOPY | - |
dc.subject | RESOLUTION | - |
dc.subject | SYSTEMS | - |
dc.subject | ORGANS | - |
dc.title | BrainFilm, a novel technique for physical compression of 3D brain slices for efficient image acquisition and post-processing | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, June Hoan | - |
dc.contributor.affiliatedAuthor | Kwag, Jeehyun | - |
dc.contributor.affiliatedAuthor | Kim, Hyun | - |
dc.contributor.affiliatedAuthor | Sun, Woong | - |
dc.identifier.doi | 10.1038/s41598-018-26776-9 | - |
dc.identifier.scopusid | 2-s2.0-85048128961 | - |
dc.identifier.wosid | 000434011100018 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.8 | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 8 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | 3-DIMENSIONAL VISUALIZATION | - |
dc.subject.keywordPlus | WHOLE-BODY | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | MICROSCOPY | - |
dc.subject.keywordPlus | RESOLUTION | - |
dc.subject.keywordPlus | SYSTEMS | - |
dc.subject.keywordPlus | ORGANS | - |
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