Multifunctionalized Reduced Graphene Oxide Biosensors for Simultaneous Monitoring of Structural Changes in Amyloid-40
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, Dahye | - |
dc.contributor.author | Kim, Jinsik | - |
dc.contributor.author | Chae, Myung-Sic | - |
dc.contributor.author | Lee, Wonseok | - |
dc.contributor.author | Yang, Seung-Hoon | - |
dc.contributor.author | Kim, YoungSoo | - |
dc.contributor.author | Kim, Seung Min | - |
dc.contributor.author | Lee, Jin San | - |
dc.contributor.author | Lee, Jeong Hoon | - |
dc.contributor.author | Choi, Jungkyu | - |
dc.contributor.author | Yoon, Dae Sung | - |
dc.contributor.author | Hwang, Kyo Seon | - |
dc.date.accessioned | 2021-09-02T10:34:38Z | - |
dc.date.available | 2021-09-02T10:34:38Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2018-06 | - |
dc.identifier.issn | 1424-8220 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/75015 | - |
dc.description.abstract | Determination of the conformation (monomer, oligomer, or fibril) of amyloid peptide aggregates in the human brain is essential for the diagnosis and treatment of Alzheimer's disease (AD). Accordingly, systematic investigation of amyloid conformation using analytical tools is essential for precisely quantifying the relative amounts of the three conformations of amyloid peptide. Here, we developed a reduced graphene oxide (rGO) based multiplexing biosensor that could be used to monitor the relative amounts of the three conformations of various amyloid- 40 (A40) fluids. The electrical rGO biosensor was composed of a multichannel sensor array capable of individual detection of monomers, oligomers, and fibrils in a single amyloid fluid sample. From the performance test of each sensor, we showed that this method had good analytical sensitivity (1 pg/mL) and a fairly wide dynamic range (1 pg/mL to 10 ng/mL) for each conformation of A40. To verify whether the rGO biosensor could be used to evaluate the relative amounts of the three conformations, various amyloid solutions (monomeric A40, aggregated A40, and disaggregated A40 solutions) were employed. Notably, different trends in the relative amounts of the three conformations were observed in each amyloid solution, indicating that this information could serve as an important parameter in the clinical setting. Accordingly, our analytical tool could precisely detect the relative amounts of the three conformations of A40 and may have potential applications as a diagnostic system for AD. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | ALZHEIMERS-DISEASE | - |
dc.subject | CEREBROSPINAL-FLUID | - |
dc.subject | BETA-PEPTIDE | - |
dc.subject | TAU | - |
dc.subject | CSF | - |
dc.subject | AGGREGATION | - |
dc.subject | FIBRILS | - |
dc.subject | PROTEIN | - |
dc.subject | BRAIN | - |
dc.subject | NEURODEGENERATION | - |
dc.title | Multifunctionalized Reduced Graphene Oxide Biosensors for Simultaneous Monitoring of Structural Changes in Amyloid-40 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Jungkyu | - |
dc.contributor.affiliatedAuthor | Yoon, Dae Sung | - |
dc.identifier.doi | 10.3390/s18061738 | - |
dc.identifier.scopusid | 2-s2.0-85047861362 | - |
dc.identifier.wosid | 000436774300072 | - |
dc.identifier.bibliographicCitation | SENSORS, v.18, no.6 | - |
dc.relation.isPartOf | SENSORS | - |
dc.citation.title | SENSORS | - |
dc.citation.volume | 18 | - |
dc.citation.number | 6 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Engineering, Electrical & Electronic | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | ALZHEIMERS-DISEASE | - |
dc.subject.keywordPlus | CEREBROSPINAL-FLUID | - |
dc.subject.keywordPlus | BETA-PEPTIDE | - |
dc.subject.keywordPlus | TAU | - |
dc.subject.keywordPlus | CSF | - |
dc.subject.keywordPlus | AGGREGATION | - |
dc.subject.keywordPlus | FIBRILS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | NEURODEGENERATION | - |
dc.subject.keywordAuthor | reduced graphene oxide(rGO) | - |
dc.subject.keywordAuthor | Alzheimer&apos | - |
dc.subject.keywordAuthor | s disease | - |
dc.subject.keywordAuthor | amyloid beta | - |
dc.subject.keywordAuthor | biosensor | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.