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Quantification of focal adhesion dynamics of cell movement based on cell-induced collagen matrix deformation using second-harmonic generation microscopy

Authors
Kang, Yong GukJang, HwanseokYang, Taeseok DanielNotbohm, JacobChoi, YoungwoonPark, YongdooKim, Beop-Min
Issue Date
6월-2018
Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
Keywords
second-harmonic generation; focal adhesion; matrix deformation; cell migration; digital volume correlation
Citation
JOURNAL OF BIOMEDICAL OPTICS, v.23, no.6
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF BIOMEDICAL OPTICS
Volume
23
Number
6
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/75018
DOI
10.1117/1.JBO.23.6.065001
ISSN
1083-3668
Abstract
Mechanical interactions of living cells with the surrounding environment via focal adhesion (FA) in three dimensions (3-D) play a key role in dynamic biological events, such as tissue regeneration, wound healing, and cancer invasion. Recently, several methods for observing 3-D cell-extracellular matrix (ECM) interactions have been reported, lacking solid and quantitative analysis on the dynamics of the physical interaction between the cell and the ECM. We measured the submicron displacements of ECM deformation in 3-D due to protrusion-retraction dynamics during cell migration, using second-harmonic generation without labeling the matrix structures. We then quantitatively analyzed the mechanical deformation between the ECM and the cells based on spatiotemporal volumetric correlations. The greatest deformations within the collagen matrix were found to occur at sites of colocalization of the FA site-related proteins vinculin and actin, which confirms that FA sites play a critical role in living cells within the ECM as a point for adhesion, traction, and migration. We believe that this modality can be used in studies of cell-ECM interaction during angiogenesis, wound healing, and metastasis. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License.
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