Pluronic/Heparin Nanoparticles for Chemo-Photodynamic Combination Cancer Therapy through Photoinduced Caspase-3 Activation
- Authors
- Khaliq, Nisar Ul; Park, Dal Yong; Lee, Hye Jin; Oh, Keun Sang; Seo, Jae Hong; Kim, Sang Yoon; Hwang, Chang Soon; Lim, Tae-Hong; Yuk, Soon Hong
- Issue Date
- 6월-2018
- Publisher
- AMER CHEMICAL SOC
- Keywords
- apoptosis; photodynamic therapy; prodrug; combination therapy; Pluronic/heparin nanoparticles
- Citation
- ACS APPLIED NANO MATERIALS, v.1, no.6, pp.2943 - 2952
- Indexed
- SCOPUS
- Journal Title
- ACS APPLIED NANO MATERIALS
- Volume
- 1
- Number
- 6
- Start Page
- 2943
- End Page
- 2952
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/75461
- DOI
- 10.1021/acsanm.8b00572
- ISSN
- 2574-0970
- Abstract
- The Pluronic/heparin nanoparticles (NPs) were prepared for chemo-photodynamic combination cancer therapy through photoinduced caspase-3 activation. Doxorubicin (DOX) prodrug and methylene blue (MB) were assembled into a single structure by electrostatic interaction with heparin and further loaded into the NPs. MB is a photosensitizer, which can be used for photodynamic therapy, which can elicit cell death by producing reactive oxygen species (ROS). The DOX prodrug was DOX conjugated to a labile tetrapeptide (DEVD) by caspase-3 upon apoptosis. ROS was measured to verify ROS-mediated apoptosis. Membrane integrity test and flow cytometry analysis were performed to examine induced apoptosis. The tumor targeting and the in vivo antitumor efficacy were measured to evaluate chemo-photodynamic combination cancer therapy in tumor-bearing mice.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
- College of Pharmacy > Department of Pharmaceutical Science > 1. Journal Articles
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