Tenacibaculum todarodis sp nov., isolated from a squid

Abstract

A Gram-stain-negative, rod-shaped, aerobic bacterial strain, designated LPB0136(T), was isolated from a squid Todarodes pacificus, caught in the East Sea, off Korea. LPB0136(T) contained a circular chromosome of 3.02 Mb with a DNA G+C content of 30.7 mol%. The genome included 2726 protein-coding genes and three copies of rRNA operons. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this isolate represents a member of the genus Tenacibaculum with an independent genomic species status, showing sequence similarities of 95.9% to Tenacibaculum aestuarii SMK-4(T) and Tenacibaculum caenipelagi HJ-26M(T). The detected respiratory quinone (MK-6) and major polar lipid phosphatidylethanolamine) were similar to the chemotaxonomic profile of other species of the genus Tenacibaculum. The major cellular fatty acids profile (iso-C-15 (: 0), iso-C-15 : 0 3-OH and iso-C(15 : 0)G) was also similar to those of members of genus Tenacibaculum, but the contents and amounts differed from those of closely related neighbours. Many biochemical and physiological characteristics also distinguished the isolate from other species within the genus Tenacibaculum. On the basis of the pholyphasic taxonomic data determined in this study, strain LPB0136(T) represents a novel species of the genus Tenacibaculum, for which the name Tenacibaculum todarodis sp. nov. is proposed. The type strain is LPB0136(T) (=KACC 18887(T) = JCM 31564(T)).