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Expression and deletion analyses of cspE encoding cold-shock protein E in Acinetobacter oleivorans DR1

Authors
Kim, JisunHa, SunheePark, Woojun
Issue Date
5월-2018
Publisher
ELSEVIER SCIENCE BV
Keywords
Cold-shock protein; CspE; CspA; Soil bacteria; Acinetobacter; Transcription
Citation
RESEARCH IN MICROBIOLOGY, v.169, no.4-5, pp.244 - 253
Indexed
SCIE
SCOPUS
Journal Title
RESEARCH IN MICROBIOLOGY
Volume
169
Number
4-5
Start Page
244
End Page
253
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/76054
DOI
10.1016/j.resmic.2018.04.011
ISSN
0923-2508
Abstract
Six genes encoding cold-shock-like proteins, including cspE, are contained within the genome of Acinetobacter oleivorans DR1. All six genes are similar in size as well as amino acid identity, but appear to be differentially regulated under stressful conditions. Four of these genes (cspA, cspB, cspC and cspE) were functionally important during cold shock because of their gradual upregulation during a temperature decrease under our assay conditions. cspE also showed higher expression during alkane degradation and antibiotic exposure. The transcriptional start site of the cspE gene was determined using 5' rapid amplification of complementary DNA ends. Next, promoter analysis using numerous constructed gfp reporter strains containing deleted fragments of cspE upstream regions identified possible 5' untranslated region (UTR) cis-DNA elements that could be involved in modulating cspE expression. Deletion of cspE led to a growth defect and enhanced biofilm formation, but only at a low temperature. Collectively, our findings show the importance of CspE during cold shock, dynamic regulation of cspE expression under various stressful conditions and a possible 5'-UTR cis-DNA element for regulation of cspE expression. These data provide molecular insight into cspE gene expression during cold-shock adaptation in soil bacteria. (C) 2018 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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생명과학대학 (환경생태공학부)
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