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A highly permselective electrochemical glucose sensor using red blood cell membrane

Authors
Kim, InsuKwon, DohyungLee, DongtakLee, Tae HoonLee, Jeong HoonLee, GyudoYoon, Dae Sung
Issue Date
15-4월-2018
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Keywords
Electrochemical analysis; Glucose biosensor; Glucose dehydrogenase; Red blood cell membrane; Glucose transporter-1; Permselectivity
Citation
BIOSENSORS & BIOELECTRONICS, v.102, pp.617 - 623
Indexed
SCIE
SCOPUS
Journal Title
BIOSENSORS & BIOELECTRONICS
Volume
102
Start Page
617
End Page
623
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/76132
DOI
10.1016/j.bios.2017.12.002
ISSN
0956-5663
Abstract
We developed an electrochemical enzymatic biosensor coated with red blood cell membrane (RBCM) for highly selective glucose measurement. The RBCM containing its membrane proteins (glucose transporter-1) was extracted from a human red blood cell (RBC), and then placed on an established enzymatic glucose sensor by the vesicle fission method. In the newly fabricated glucose sensor, the RBCM plays the role in the diffusion barrier of preventing the penetration of interfering molecules, while in contrast making glucose molecules permeable. We employed scanning electron microscopy and atomic force microscopy to analyze the RBCM diffusion barriers. The performance of our glucose sensors with diffusion barriers was tested regarding selectivity to glucose against other interfering molecules, such as ascorbic acid, uric acid, and galactose. Remarkably, employing the RBCM significantly improved the selectivity and accuracy of the glucose measurement, even under human serum, compared to the uncoated sensors. This result implies that the RBCM serves well as a highly permselective layer to glucose molecules, and a diffusion barrier to other molecules.
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Graduate School > Department of Biotechnology and Bioinformatics > 1. Journal Articles
Graduate School > Department of Bioengineering > 1. Journal Articles

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