Comparison of subchronic immunotoxicity of four different types of aluminum-based nanoparticles

Abstract

Nanoparticles (NPs) have recently emerged as an inhalable pollutant, owing to their applications, aluminum-based NPs (Al-NPs) have been prioritized for toxicity testing. In the current study, we compared the pulmonary biopersistence and subsequent toxicity of four different types of Al-NPs (two rod-type aluminum oxide NPs [AlONPs] with different aspect ratios [short (S)- and long (L)-AlONPs], spherical aluminum cerium oxide NPs [AlCeO3, AlCeONPs] and spherical -aluminum oxide hydroxide nanoparticles [AlOOHNPs]) 13weeks after a single intratracheal instillation, considering the importance of their properties in their toxicity. We found that the pulmonary biopersistence of Al-NPs was strengthened by a high aspect ratio in the rod-type AlONPs and by the presence of hydroxyl groups in the spherical-type Al-NPs. The highest toxicity was observed in the mice treated with AlOOHNPs, which showed low biostability. More importantly, we identified that the commercially available AlCeONPs were Al2O3-coated CeO2 NPs, but not AlCeO3 NPs, although they have been sold under the trade name of AlCeONPs. In conclusion, the aspect ratio and biostability may be important factors in the determination of the biopersistence of NPs and the subsequent biological response. In addition, the physicochemical properties of NPs should be examined in detail before their release into the market to prevent unexpected adverse health effects. We compared the pulmonary biopersistence and subsequent toxicity of four different types of Al-NPs [two rod-type AlONPs with different aspect ratios (short- and long-AlONPs), spherical aluminum cerium oxide nanoparticles (AlCeO3, AlCeONPs), and spherical -aluminum oxide hydroxide nanoparticles (AlOOHNPs)] 13 wks after a single instillation. The pulmonary biopersistence was strengthened by a high aspect ratio in the rod-types and by the presence of hydroxyl groups in the spherical-types, and the toxicity was highest in AlOOHNPs-treated mice due to low biostability.