A novel beta-glucosidase from Saccharophagus degradans 2-40(T) for the efficient hydrolysis of laminarin from brown macroalgae

Abstract

Background: Laminarin is a potential biomass feedstock for the production of glucose, which is the most preferable fermentable sugar in many microorganisms by which it can be converted to biofuels and bio-based chemicals. Also, laminarin is a good resource as functional materials because it consists of beta-1,3-glucosidic linkages in its backbone and beta-1,6-glucosidic linkages in its branches so that its oligosaccharides driven from laminarin have a variety of biological activities. It is industrially important to be able to produce laminarioligosaccharides as well as glucose from laminarin by a single enzyme because the enzyme cost accounts for a large part of bio-based products. In this study, we investigated the industrial applicability of Bgl1B, a unique beta-glucosidase from Saccharophagus degradans 2-40(T), belonging to the glycoside hydrolase family 1 (GH1) by characterizing its activity of hydrolyzing laminarin under various conditions. Results: Bgl1B was cloned and overexpressed in Escherichia coli from S. degradans 2-40(T), and its enzymatic activity was characterized. Similar to most of beta-glucosidases in GH1, Bgl1B was able to hydrolyze a variety of disaccharides having different beta-linkages, such as laminaribiose, cellobiose, gentiobiose, lactose, and agarobiose, by cleaving beta-1,3-, beta-1,4-, and beta-1,6-glycosidic linkages. However, Bgl1B showed the highest specific activity toward laminaribiose with a beta-1,3-glycosidic linkage. In addition, it was able to hydrolyze laminarin, one of the major polysaccharides in brown macroalgae, into glucose with a conversion yield of 75% of theoretical maximum. Bgl1B also showed transglycosylation activity by producing oligosaccharides from laminarin and laminaribiose under a high mass ratio of substrate to enzyme. Furthermore, Bgl1B was found to be psychrophilic, exhibiting relative activity of 59-85% in the low-temperature range of 2-20 degrees C. Conclusions: Bgl1B can directly hydrolyze laminarin into glucose with a high conversion yield without leaving any oligosaccharides. Bgl1B can exhibit high enzymatic activity in a broad range of low temperatures (2-20 degrees C), which is advantageous for establishing energy-efficient bioprocesses. In addition, under high substrate to enzyme ratios, Bgl1B can produce high-value laminarioligosaccharides via its transglycosylation activity. These results show that Bgl1B can be an industrially important enzyme for the production of biofuels and bio-based chemicals from brown macroalgae.