Evaluation and control of miRNA-like off-target repression for RNA interference
DC Field | Value | Language |
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dc.contributor.author | Seok, Heeyoung | - |
dc.contributor.author | Lee, Haejeong | - |
dc.contributor.author | Jang, Eun-Sook | - |
dc.contributor.author | Chi, Sung Wook | - |
dc.date.accessioned | 2021-09-02T14:45:22Z | - |
dc.date.available | 2021-09-02T14:45:22Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-03 | - |
dc.identifier.issn | 1420-682X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/77252 | - |
dc.description.abstract | RNA interference (RNAi) has been widely adopted to repress specific gene expression and is easily achieved by designing small interfering RNAs (siRNAs) with perfect sequence complementarity to the intended target mRNAs. Although siRNAs direct Argonaute (Ago), a core component of the RNA-induced silencing complex (RISC), to recognize and silence target mRNAs, they also inevitably function as microRNAs (miRNAs) and suppress hundreds of off-targets. Such miRNA-like off-target repression is potentially detrimental, resulting in unwanted toxicity and phenotypes. Despite early recognition of the severity of miRNA-like off-target repression, this effect has often been overlooked because of difficulties in recognizing and avoiding off-targets. However, recent advances in genome-wide methods and knowledge of Ago-miRNA target interactions have set the stage for properly evaluating and controlling miRNA-like off-target repression. Here, we describe the intrinsic problems of miRNA-like off-target effects caused by canonical and noncanonical interactions. We particularly focus on various genome-wide approaches and chemical modifications for the evaluation and prevention of off-target repression to facilitate the use of RNAi with secured specificity. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER BASEL AG | - |
dc.subject | BINDING-SITES | - |
dc.subject | SEED REGION | - |
dc.subject | IN-VIVO | - |
dc.subject | SYSTEMATIC IDENTIFICATION | - |
dc.subject | CHEMICAL-MODIFICATIONS | - |
dc.subject | NUCLEOTIDE RESOLUTION | - |
dc.subject | MAMMALIAN MICRORNAS | - |
dc.subject | HUMAN ARGONAUTE-2 | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | MESSENGER-RNAS | - |
dc.title | Evaluation and control of miRNA-like off-target repression for RNA interference | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Seok, Heeyoung | - |
dc.contributor.affiliatedAuthor | Chi, Sung Wook | - |
dc.identifier.doi | 10.1007/s00018-017-2656-0 | - |
dc.identifier.scopusid | 2-s2.0-85029428006 | - |
dc.identifier.wosid | 000424876800003 | - |
dc.identifier.bibliographicCitation | CELLULAR AND MOLECULAR LIFE SCIENCES, v.75, no.5, pp.797 - 814 | - |
dc.relation.isPartOf | CELLULAR AND MOLECULAR LIFE SCIENCES | - |
dc.citation.title | CELLULAR AND MOLECULAR LIFE SCIENCES | - |
dc.citation.volume | 75 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 797 | - |
dc.citation.endPage | 814 | - |
dc.type.rims | ART | - |
dc.type.docType | Review | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | BINDING-SITES | - |
dc.subject.keywordPlus | SEED REGION | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | SYSTEMATIC IDENTIFICATION | - |
dc.subject.keywordPlus | CHEMICAL-MODIFICATIONS | - |
dc.subject.keywordPlus | NUCLEOTIDE RESOLUTION | - |
dc.subject.keywordPlus | MAMMALIAN MICRORNAS | - |
dc.subject.keywordPlus | HUMAN ARGONAUTE-2 | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | MESSENGER-RNAS | - |
dc.subject.keywordAuthor | Off-target effects | - |
dc.subject.keywordAuthor | miRNA seed sites | - |
dc.subject.keywordAuthor | Noncanonical target sites | - |
dc.subject.keywordAuthor | Ago HITS-CLIP | - |
dc.subject.keywordAuthor | RNA-Seq | - |
dc.subject.keywordAuthor | Ribo-Seq | - |
dc.subject.keywordAuthor | Chemical modification | - |
dc.subject.keywordAuthor | Abasic pivot | - |
dc.subject.keywordAuthor | RNAi therapeutics | - |
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