Arsenic trioxide attenuates STAT-3 activity and epithelial-mesenchymal transition through induction of SHP-1 in gastric cancer cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Sung Ho | - |
dc.contributor.author | Yoo, Hyo Soon | - |
dc.contributor.author | Joo, Moon Kyung | - |
dc.contributor.author | Kim, Taehyun | - |
dc.contributor.author | Park, Jong-Jae | - |
dc.contributor.author | Lee, Beom Jae | - |
dc.contributor.author | Chun, Hoon Jai | - |
dc.contributor.author | Lee, Sang Woo | - |
dc.contributor.author | Bak, Young-Tae | - |
dc.date.accessioned | 2021-09-02T15:00:15Z | - |
dc.date.available | 2021-09-02T15:00:15Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-02-06 | - |
dc.identifier.issn | 1471-2407 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/77377 | - |
dc.description.abstract | Background: We investigated the effect of arsenic trioxide (ATO) for inhibition of signal transducer and activator of transcription 3 (STAT3) and epithelial-mesenchymal transition (EMT) in gastric cancer cells, and the role of SH2 domain-containing phosphatase-1 (SHP-1) during this process. Methods: We used AGS cells, which showed minimal SHP-1 expression and constitutive STAT3 expression. After treatment of ATO, cellular migration and invasion were assessed by using wound closure assay, Matrigel invasion assay and 3-D culture invasion assay. To validate the role of SHP-1, pervanadate, a pharmacologic phosphatase inhibitor, and SHP-1 siRNA were used. Xenograft tumors were produced, and ATO or pervanadate were administered via intraperitoneal (IP) route. Results: Treatment of ATO 5 and 10 mu M significantly decreased cellular migration and invasion in a dose-dependent manner. Western blot showed that ATO upregulated SHP-1 expression and downregulated STAT3 expression, and immunofluorescence showed upregulation with E-cadherin (epithelial marker) and downregulation of Snail1 (mesenchymal marker) expression by ATO treatment. Anti-migration and invasion effect and modulation of SHP-1/STAT3 axis by ATO were attenuated by pervanadate or SHP-1/siRNA. IP injection of ATO significantly decreased the xenograft tumor volume and upregulated SHP-1 expression, which were attenuated by co-IP injection of pervanadate. Conclusion: Our data suggest that ATO inhibits STAT3 activity and EMT process by upregulation of SHP-1 in gastric cancer cells. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.subject | TYROSINE-PHOSPHATASE 1 | - |
dc.subject | HEPATOCELLULAR-CARCINOMA | - |
dc.subject | TARGETING STAT3 | - |
dc.subject | INHIBITION | - |
dc.subject | SORAFENIB | - |
dc.subject | APOPTOSIS | - |
dc.subject | PROGRESSION | - |
dc.subject | ACTIVATION | - |
dc.subject | RESISTANCE | - |
dc.title | Arsenic trioxide attenuates STAT-3 activity and epithelial-mesenchymal transition through induction of SHP-1 in gastric cancer cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Joo, Moon Kyung | - |
dc.contributor.affiliatedAuthor | Park, Jong-Jae | - |
dc.contributor.affiliatedAuthor | Lee, Beom Jae | - |
dc.contributor.affiliatedAuthor | Chun, Hoon Jai | - |
dc.contributor.affiliatedAuthor | Lee, Sang Woo | - |
dc.contributor.affiliatedAuthor | Bak, Young-Tae | - |
dc.identifier.doi | 10.1186/s12885-018-4071-9 | - |
dc.identifier.scopusid | 2-s2.0-85041428505 | - |
dc.identifier.wosid | 000424775700012 | - |
dc.identifier.bibliographicCitation | BMC CANCER, v.18 | - |
dc.relation.isPartOf | BMC CANCER | - |
dc.citation.title | BMC CANCER | - |
dc.citation.volume | 18 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | TYROSINE-PHOSPHATASE 1 | - |
dc.subject.keywordPlus | HEPATOCELLULAR-CARCINOMA | - |
dc.subject.keywordPlus | TARGETING STAT3 | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | SORAFENIB | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | PROGRESSION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordAuthor | Arsenic trioxide | - |
dc.subject.keywordAuthor | Epithelial-mesenchymal transition | - |
dc.subject.keywordAuthor | SH2-containing protein tyrosine phosphatase 1 | - |
dc.subject.keywordAuthor | Signal transducer and activator of transcription 3 | - |
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