Investigation of dermal toxicity of ionic liquids in monolayer-cultured skin cells and 3D reconstructed human skin models
DC Field | Value | Language |
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dc.contributor.author | Hwang, Jee-hyun | - |
dc.contributor.author | Park, Hyeonji | - |
dc.contributor.author | Choi, Dal Woong | - |
dc.contributor.author | Nam, Ki Taek | - |
dc.contributor.author | Lim, Kyung-Min | - |
dc.date.accessioned | 2021-09-02T15:54:42Z | - |
dc.date.available | 2021-09-02T15:54:42Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-02 | - |
dc.identifier.issn | 0887-2333 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/77864 | - |
dc.description.abstract | Ionic liquids have gained increasing attention in the chemical industry as potential green substitutes for traditional solvents. However, little is known about toxicity of ionic liquids on the skin, a major exposure portal to toxic substances. Here, we evaluated dermal toxicity of ionic liquids using human keratinocyte and fibroblast cell line, 3D reconstructed human epidermis, and full-thickness model to investigate underlying mechanisms. Cytotoxicity of ionic liquids was evaluated for representative anions, [TFSI], [PF6], [BF4], and [DCA], as well as for cations, [EMIM], [BMPY], [TBA] and [Zn], in human keratinocyte cell line, HaCaT, and human dermal fibroblasts. In our results, significant cytotoxicity was induced by ionic liquids with [TFSI] in both cell lines. Notably, cytotoxicity of [TFSI] containing ionic liquids was comparable to xylene, a toxic conventional organic solvent. Fluorescent and flow cytometric analysis revealed that [TFSI]-exposed cells underwent necrotic cell death. Reactive oxygen species (ROS) was increased while the amount of glutathione was decreased by [TFSI] in dose-dependent manner, which was reversed by antioxidant, N-acetyleysteine. In 3D reconstructed human epidermis and full-thickness model, a single application of [TFSI] induced toxicity although it was minimal and largely limited to epidermal layer. Collectively, these results demonstrated potential dermal toxicity of ionic liquids. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.subject | IN-VITRO | - |
dc.subject | GREEN SOLVENT | - |
dc.subject | CYTOTOXICITY | - |
dc.title | Investigation of dermal toxicity of ionic liquids in monolayer-cultured skin cells and 3D reconstructed human skin models | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Dal Woong | - |
dc.identifier.doi | 10.1016/j.tiv.2017.09.025 | - |
dc.identifier.scopusid | 2-s2.0-85031099821 | - |
dc.identifier.wosid | 000422811300023 | - |
dc.identifier.bibliographicCitation | TOXICOLOGY IN VITRO, v.46, pp.194 - 202 | - |
dc.relation.isPartOf | TOXICOLOGY IN VITRO | - |
dc.citation.title | TOXICOLOGY IN VITRO | - |
dc.citation.volume | 46 | - |
dc.citation.startPage | 194 | - |
dc.citation.endPage | 202 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | GREEN SOLVENT | - |
dc.subject.keywordPlus | CYTOTOXICITY | - |
dc.subject.keywordAuthor | Ionic liquid | - |
dc.subject.keywordAuthor | Bis(trifluoromethanesulfonyl)imide, TFSI | - |
dc.subject.keywordAuthor | Dermal toxicity | - |
dc.subject.keywordAuthor | ROS | - |
dc.subject.keywordAuthor | 3D skin model | - |
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