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Production of xylanase from a novel engineered Pichia pastoris and application to enzymatic hydrolysis process for biorefinery

Authors
Kim, Chan KyumChoi, Han SukLee, Sang JunLee, Ja HyunLee, Ju HunYoo, Hah YoungHan, Sung OkKim, Seung Wook
Issue Date
2월-2018
Publisher
ELSEVIER SCI LTD
Keywords
Biorefinery; Cellulomonas flavigena; Enzymatic hydrolysis; Pichia pastoris; Xylanase
Citation
PROCESS BIOCHEMISTRY, v.65, pp.130 - 135
Indexed
SCIE
SCOPUS
Journal Title
PROCESS BIOCHEMISTRY
Volume
65
Start Page
130
End Page
135
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/77883
DOI
10.1016/j.procbio.2017.11.001
ISSN
1359-5113
Abstract
In this study, the xylanase gene from Cellulomonas flavigena KCTC 9104 was cloned into pPICZ alpha B and expressed in Pichia pastoris X-33. An extracellular endo-1,4-beta-xylanase was produced by novel engineered P. pastoris (rXynCf) and purified by Ni-NTA affinity column. Characterization of rXynCf was performed and results are as follows: 38 kDa molecular weight, 55 degrees C optimum temperature and optimum pH of 6. Under the conditions, the K-m and V-max of rXynCf were 3.6 +/- 0.08 mg/mL and 4505 +/- 52 mu mol/min mg, respectively. rXynCf was applied in enzymatic hydrolysis process for sugars production from lignocellulosic biomass. Empty fruit bunch (EFB) was selected as a feedstock, and the total sugars conversion was found to be 3.8%, meanwhile the conversion by alkaline pretreatment improved approximately 16-fold (61.1%). In addition, rXynCf shows similar xylose conversion to commercial xylanase. Therefore, due to its properties, rXynCf has considerable potential in biorefinery applications.
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Graduate School > Department of Biotechnology > 1. Journal Articles
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