Preventive effect of anti-VacA egg yolk immunoglobulin (IgY) on Helicobacter pylori-infected mice
DC Field | Value | Language |
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dc.contributor.author | Hong, Kyung Sook | - |
dc.contributor.author | Ki, Mi-Ran | - |
dc.contributor.author | Ullah, H. M. Arif | - |
dc.contributor.author | Lee, Eun-Joo | - |
dc.contributor.author | Kim, Yong Deuk | - |
dc.contributor.author | Chung, Myung-Jin | - |
dc.contributor.author | Elfadl, Ahmed K. | - |
dc.contributor.author | Park, Jin-Kyu | - |
dc.contributor.author | Jeong, Kyu-Shik | - |
dc.date.accessioned | 2021-09-02T16:12:50Z | - |
dc.date.available | 2021-09-02T16:12:50Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2018-01-08 | - |
dc.identifier.issn | 0264-410X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/77995 | - |
dc.description.abstract | Background: Helicobacter pylori, a gram-negative bacterium, is the causative agent of gastric disorders and gastric cancer in the human stomach. Vacuolating cytotoxin A (VacA) is among the multi-effect protein toxins released by H. pylori that enables its persistence in the human stomach. Methods: To evaluate the effect of anti-VacA egg yolk immunoglobulin (anti-VacA IgY) on H. pylori infection, a highly specific anti-VacA IgY was produced from egg yolks of hens immunized with a mixture of two purified recombinant VacAs. Female C57BL/6 mice were supplemented anti-VacA IgY daily with drinking water for 2 weeks before and 4 weeks after H. pylori ATCC 43504 inoculation. Anti-VacA IgY recognized both native and denatured structures of VacA by enzyme-linked immunosorbent assay and immunoblotting analyses, respectively. Results: Oral administration of anti-VacA IgYs significantly (p < .05) reduced the serum levels of anti-H. pylori antibodies compared to those in the H. pylori-infected, untreated group. The reduction in the immune response was accompanied by a significant (p < .05) decrease in eosinophilic infiltration of the stomach in anti-VacA IgY treated group compared to other groups. Concomitantly, H. pylori-induced histological changes and H. pylori antigen-positivity in gastric tissues were decreased significantly (p < .05) in anti-VacA IgY treated group similar to the control group. Conclusions: Oral administration of anti-VacA IgY is correlated with a protective effect against H. pylori colonization and induced histological changes in gastric tissues. Our experimental study has proved that it is expected to be a new drug candidate of Hp infection by further study. (C) 2017 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | VACUOLATING CYTOTOXIN VACA | - |
dc.subject | VACCINE DEVELOPMENT | - |
dc.subject | HOST-CELLS | - |
dc.subject | MAST-CELLS | - |
dc.subject | ANTIBODIES | - |
dc.subject | MODEL | - |
dc.subject | IMMUNOGENICITY | - |
dc.subject | EOSINOPHILS | - |
dc.subject | GASTRITIS | - |
dc.subject | SAFETY | - |
dc.title | Preventive effect of anti-VacA egg yolk immunoglobulin (IgY) on Helicobacter pylori-infected mice | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ki, Mi-Ran | - |
dc.identifier.doi | 10.1016/j.vaccine.2017.11.082 | - |
dc.identifier.scopusid | 2-s2.0-85040765942 | - |
dc.identifier.wosid | 000429509600006 | - |
dc.identifier.bibliographicCitation | VACCINE, v.36, no.3, pp.371 - 380 | - |
dc.relation.isPartOf | VACCINE | - |
dc.citation.title | VACCINE | - |
dc.citation.volume | 36 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 371 | - |
dc.citation.endPage | 380 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | VACUOLATING CYTOTOXIN VACA | - |
dc.subject.keywordPlus | VACCINE DEVELOPMENT | - |
dc.subject.keywordPlus | HOST-CELLS | - |
dc.subject.keywordPlus | MAST-CELLS | - |
dc.subject.keywordPlus | ANTIBODIES | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | IMMUNOGENICITY | - |
dc.subject.keywordPlus | EOSINOPHILS | - |
dc.subject.keywordPlus | GASTRITIS | - |
dc.subject.keywordPlus | SAFETY | - |
dc.subject.keywordAuthor | Helicobacter pylori | - |
dc.subject.keywordAuthor | Anti-VacA | - |
dc.subject.keywordAuthor | Vaccine | - |
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