Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Development of a multiplexed opsonophagocytic killing assay (MOPA) for group B Streptococcus

Authors
Choi, Min JooNoh, Ji YunCheong, Hee JinKim, Woo JooLin, Shun-MeiZhi, YongLim, Jae HyangLim, SangyongSeo, Ho SeongSong, Joon Young
Issue Date
2018
Publisher
TAYLOR & FRANCIS INC
Keywords
capsular polysaccharide; immunoassay; opsonophagocytic killing assay; Streptococcus agalactiae
Citation
HUMAN VACCINES & IMMUNOTHERAPEUTICS, v.14, no.1, pp.67 - 73
Indexed
SCIE
SCOPUS
Journal Title
HUMAN VACCINES & IMMUNOTHERAPEUTICS
Volume
14
Number
1
Start Page
67
End Page
73
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/80977
DOI
10.1080/21645515.2017.1377379
ISSN
2164-5515
Abstract
Group B Streptococcus (GBS) is a leading cause of sepsis in infants as well as chorioamnionitis in pregnant women. Opsonophagocytic killing assays (OPAs) are an essential technique in vaccine studies of encapsulated bacteria for estimating serotype-specific functional antibody levels in vitro. Here, we developed a three-fold multiplexed OPA (MOPA) to enable practical, large-scale assessment of GBS vaccine immunogenicity, including against serotypes Ia, III, and V. First, three target bacteria strains resistant to streptomycin, spectinomycin, or kanamycin were generated by natural selection through exposure to increasing antibiotic concentrations. Since a high level of nonspecific killing (NSK) of serotype V was observed in a 12.5% baby rabbit complement (BRC) solution, the BRC concentration was optimized. The final GBS-MOPA BRC concentration was 9%, which resulted in less than 20% NSK. The specificity was measured by preabsorbing serum with inactivated GBS. The opsonic index (OI) of preabsorbed serum with the homologous serotype GBS was significantly reduced in all three serotypes tested. The accuracy of the MOPA was compared with that of a single OPA (SOPA) with 35 serum samples. The OIs of the MOPA correlated well with those of the SOPA, and the r(2) values were higher than 0.950 for all three serotypes. The precision of the MOPA assay was assessed in five independent experiments with five serum samples. The inter-assay precision of the GBS-MOPA was 12.5% of the average coefficient of variation. This is the first report to develop and standardize a GBS-MOPA, which will be useful for GBS vaccine development and evaluation.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Medicine > Department of Medical Science > 1. Journal Articles
Graduate School > Department of Biomedical Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Choeng, Hee Jin photo

Choeng, Hee Jin
의과대학 (의학과)
Read more

Altmetrics

Total Views & Downloads

BROWSE