Design of bio-inspired silica-encapsulated protein A for improved immunoprecipitation assays
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Ki Sung | - |
dc.contributor.author | Ki, Mi-Ran | - |
dc.contributor.author | Yeo, Ki Saek | - |
dc.contributor.author | Choi, Jung Hoon | - |
dc.contributor.author | Pack, Seung Pil | - |
dc.date.accessioned | 2021-09-02T21:52:50Z | - |
dc.date.available | 2021-09-02T21:52:50Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-12-15 | - |
dc.identifier.issn | 1369-703X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/81161 | - |
dc.description.abstract | Staphylococcus aureus Protein A (SpA) has a high affinity to the Fc region of antibodies (Abs), and SpA-immobilized matrices are widely used for Ab purification or immunoprecipitation (IP) assays. Here, we employed a bio-inspired silica-encapsulation method to improve the Ab-binding ability of an SpA-immobilized matrix. Two silica-forming peptides (SFPs), namely R5 and EctP1, were separately introduced at the C-terminus of SpA to generate two recombinant fusion proteins (SpA-SFPs) with auto-silicifying abilities. When SpA-SFPs were employed as Ab-binders on a solid surface (96-well plate), they showed an effective Ab-binding ability and a better performance than intact SpA. A high binding ability was observed even when an SFP-mediated SpA silica matrix (SpA-SFP@SiO2) was prepared. SpA-SFP@SiO2 showed a higher performance than commercially obtained SpA-Agarose particles and no loss of matrices. Moreover, in IP assays, SpA-SFP@SiO2 showed an approximately 300% higher precipitation of target protein than the commercial SpA-Agarose product when a small amount of cell lysate was used. These findings demonstrated that SpA-SFP could be useful for the development of an efficient immunoassay system. (C) 2017 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | AFFINITY-CHROMATOGRAPHY | - |
dc.subject | STAPHYLOCOCCUS-AUREUS | - |
dc.subject | IGG ANTIBODIES | - |
dc.subject | BIOSILICA | - |
dc.subject | SYSTEM | - |
dc.subject | NANOPARTICLES | - |
dc.subject | CATALYSTS | - |
dc.subject | PEPTIDES | - |
dc.title | Design of bio-inspired silica-encapsulated protein A for improved immunoprecipitation assays | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ki, Mi-Ran | - |
dc.contributor.affiliatedAuthor | Pack, Seung Pil | - |
dc.identifier.doi | 10.1016/j.bej.2017.08.017 | - |
dc.identifier.scopusid | 2-s2.0-85029394657 | - |
dc.identifier.wosid | 000417008900002 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL ENGINEERING JOURNAL, v.128, pp.12 - 18 | - |
dc.relation.isPartOf | BIOCHEMICAL ENGINEERING JOURNAL | - |
dc.citation.title | BIOCHEMICAL ENGINEERING JOURNAL | - |
dc.citation.volume | 128 | - |
dc.citation.startPage | 12 | - |
dc.citation.endPage | 18 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | AFFINITY-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | STAPHYLOCOCCUS-AUREUS | - |
dc.subject.keywordPlus | IGG ANTIBODIES | - |
dc.subject.keywordPlus | BIOSILICA | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | CATALYSTS | - |
dc.subject.keywordPlus | PEPTIDES | - |
dc.subject.keywordAuthor | Staphylococcus aureus Protein A (SpA) | - |
dc.subject.keywordAuthor | Biosilica | - |
dc.subject.keywordAuthor | Encapsulation | - |
dc.subject.keywordAuthor | Immunoprecipitation (IP) | - |
dc.subject.keywordAuthor | Silica-forming peptide (SFP) | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.