Biochemical-immunological hybrid biosensor based on two-dimensional chromatography for on-site sepsis diagnosis
DC Field | Value | Language |
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dc.contributor.author | Kim, Seung-Wan | - |
dc.contributor.author | Cho, Il-Hoon | - |
dc.contributor.author | Lim, Guei-Sam | - |
dc.contributor.author | Park, Gi-Na | - |
dc.contributor.author | Paek, Se-Hwan | - |
dc.date.accessioned | 2021-09-02T21:54:20Z | - |
dc.date.available | 2021-09-02T21:54:20Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-12-15 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/81173 | - |
dc.description.abstract | A hybrid-biosensor system that can simultaneously fulfill the immunoassay for protein markers (e.g., C-reactive protein (CRP) and procalcitonin (PCT)) and the enzyme assay for metabolic substances (e.g., lactate) in the same sepsis-based sample has been devised. Such a challenge was pursued through the installation of an enzyme-reaction zone on the signal pad of the typical immuno-strip for the rapid two-dimensional (2-D)-chromatography test. To minimize the mutual interference in the hybrid assays, a pre-determined membrane site was etched in a pattern and mounted with a biochemical-reaction pad, thereby allowing a loaded sample to enter and then stay in the pad for a colored-signal production over the course of an immunoassay. By employing such a constructed system, a serum sample was analyzed according to the vertical direction flowing along the strip, which supplied lactate to the biochemical-reaction zone and then protein markers to the immunological-binding area that was pre-coated with capture antibodies. Thereafter, the enzyme-signal tracers for the immunoassay and the substrate solution were sequentially furnished using a horizontal path for the tracing of the immune complexes that were formed with CRP or PCT. The color signal that was produced from each assay was detected at a pre-determined time and quantified on a smartphone-based detector. Under the optimal conditions, the dynamic ranges for the analytes covered the respective clinical ranges, and the total coefficient of variation was between 8.6% and 13.3%. The hybrid biosensor further showed a high correlation (R-2 > 0.95) with the reference systems for the target markers. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.subject | C-REACTIVE PROTEIN | - |
dc.subject | LACTATE | - |
dc.subject | PROCALCITONIN | - |
dc.subject | ENZYME | - |
dc.subject | INDICATOR | - |
dc.subject | ASSAY | - |
dc.subject | SHOCK | - |
dc.title | Biochemical-immunological hybrid biosensor based on two-dimensional chromatography for on-site sepsis diagnosis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Paek, Se-Hwan | - |
dc.identifier.doi | 10.1016/j.bios.2017.06.032 | - |
dc.identifier.scopusid | 2-s2.0-85021060613 | - |
dc.identifier.wosid | 000407538300002 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.98, pp.7 - 14 | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 98 | - |
dc.citation.startPage | 7 | - |
dc.citation.endPage | 14 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | C-REACTIVE PROTEIN | - |
dc.subject.keywordPlus | LACTATE | - |
dc.subject.keywordPlus | PROCALCITONIN | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | INDICATOR | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | SHOCK | - |
dc.subject.keywordAuthor | Heterogeneous analytes | - |
dc.subject.keywordAuthor | Multiplexed biosensing | - |
dc.subject.keywordAuthor | Two-dimensional chromatography | - |
dc.subject.keywordAuthor | Smartphone detector | - |
dc.subject.keywordAuthor | Rapid sepsis diagnosis | - |
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