Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jae Young | - |
dc.contributor.author | Seo, Eun Seok | - |
dc.contributor.author | Kim, Hyunmin | - |
dc.contributor.author | Park, Ji-Won | - |
dc.contributor.author | Lim, Dong-Kwon | - |
dc.contributor.author | Moon, Dae Won | - |
dc.date.accessioned | 2021-09-02T21:56:18Z | - |
dc.date.available | 2021-09-02T21:56:18Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-12-13 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/81188 | - |
dc.description.abstract | We report a high spatial resolution mass spectrometry (MS) system that allows us to image live hippocampal tissue slices under open-air atmospheric pressure (AP) and ambient temperature conditions at the subcellular level. The method is based on an efficient desorption process by femtosecond (fs) laser assisted with nanoparticles and a subsequent ionization step by applying nonthermal plasma, termed AP nanoparticle and plasma assisted laser desorption ionization (AP-nanoPALDI) MS method. Combining the AP-nanoPALDI with microscopic sample scanning, MS imaging with spatial resolution of 2.9 mu m was obtained. The observed AP-nanoPALDI MS imaging clearly revealed the differences of molecular composition between the apical and basal dendrite regions of a hippocampal tissue. In addition, the AP-nanoPALDI MS imaging showed the decrease of cholesterol in hippocampus by treating with methyl beta-cyclodextrin, which exemplifies the potential of AP-nanoPALDI for live tissue imaging for various biomedical applications without any chemical pretreatment and/or labeling process. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | FLOW-ASSISTED IONIZATION | - |
dc.subject | ELECTROSPRAY-IONIZATION | - |
dc.subject | CHEMICAL-CHARACTERIZATION | - |
dc.subject | DENTATE GYRUS | - |
dc.subject | ION-SOURCE | - |
dc.subject | REAL-TIME | - |
dc.subject | AIR | - |
dc.subject | INSTRUMENTATION | - |
dc.subject | SURFACES | - |
dc.subject | LIPIDS | - |
dc.title | Atmospheric pressure mass spectrometric imaging of live hippocampal tissue slices with subcellular spatial resolution | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lim, Dong-Kwon | - |
dc.identifier.doi | 10.1038/s41467-017-02216-6 | - |
dc.identifier.scopusid | 2-s2.0-85038433296 | - |
dc.identifier.wosid | 000417884500019 | - |
dc.identifier.bibliographicCitation | NATURE COMMUNICATIONS, v.8 | - |
dc.relation.isPartOf | NATURE COMMUNICATIONS | - |
dc.citation.title | NATURE COMMUNICATIONS | - |
dc.citation.volume | 8 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | FLOW-ASSISTED IONIZATION | - |
dc.subject.keywordPlus | ELECTROSPRAY-IONIZATION | - |
dc.subject.keywordPlus | CHEMICAL-CHARACTERIZATION | - |
dc.subject.keywordPlus | DENTATE GYRUS | - |
dc.subject.keywordPlus | ION-SOURCE | - |
dc.subject.keywordPlus | REAL-TIME | - |
dc.subject.keywordPlus | AIR | - |
dc.subject.keywordPlus | INSTRUMENTATION | - |
dc.subject.keywordPlus | SURFACES | - |
dc.subject.keywordPlus | LIPIDS | - |
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