Direct Conversion of CO2 to alpha-Farnesene Using Metabolically Engineered Synechococcus elongatus PCC 7942
- Authors
- Lee, Hyun Jeong; Lee, Jiwon; Lee, Sun-Mi; Um, Youngsoon; Kim, Yunje; Sim, Sang Jun; Choi, Jong-il; Woo, Han Min
- Issue Date
- 6-12월-2017
- Publisher
- AMER CHEMICAL SOC
- Keywords
- cyanobacteria; metabolic engineering; farnesene; CO2 conversion
- Citation
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.65, no.48, pp.10424 - 10428
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
- Volume
- 65
- Number
- 48
- Start Page
- 10424
- End Page
- 10428
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/81204
- DOI
- 10.1021/acs.jafc.7b03625
- ISSN
- 0021-8561
- Abstract
- Direct conversion of carbon dioxide (CO2) to value-added chemicals by engineering of cyanobacteria has received attention as a sustainable strategy in food and chemical industries. Herein, Synechococcus elongatus PCC 7942, a model cyanobacterium, was engineered to produce alpha-farnesene from CO2. As a result of the lack of famesene synthase (FS) activity in the wild-type cyanobacterium, we metabolically engineered S. elongatus PCC 7942 to express heterologous FS from either Norway spruce or apple fruit, resulting in detectable peaks of alpha-farnesene. To enhance alpha-farnesene production, an optimized methylerythritol phosphate (MEP) pathway was introduced in the farnesene-producing strain to supply farnesyl diphosphate. Subsequent cyanobacterial culture with a dodecane overlay resulted in photosynthetic production of alpha-farnesene (4.6 +/- 0.4 mg/L in 7 days) from CO2. To the best of our knowledge, this is the first report of the photosynthetic production of alpha-farnesene from CO2 in the unicellular cyanobacterium S. elongatus PCC 7942.
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