Uniform-sized neurosphere-mediated motoneuron differentiation in microwell arrays
DC Field | Value | Language |
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dc.contributor.author | Lee, Jong Min | - |
dc.contributor.author | Moon, Joo Yoon | - |
dc.contributor.author | Shaker, Mohammed R. | - |
dc.contributor.author | Sun, Woong | - |
dc.contributor.author | Chung, Bong Geun | - |
dc.date.accessioned | 2021-09-02T22:11:27Z | - |
dc.date.available | 2021-09-02T22:11:27Z | - |
dc.date.created | 2021-06-16 | - |
dc.date.issued | 2017-12 | - |
dc.identifier.issn | 0173-0835 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/81260 | - |
dc.description.abstract | We developed the photocrosslinkable hydrogel microwell arrays for uniform-sized neurosphere-mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform-sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform-sized neurospheres harvested from microwell arrays were replated into laminin-coated substrate. In parallel, uniform-sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three-dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150m in diameter) on motoneuron differentiation, showing that the largest uniform-sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform-sized neurosphere-mediated motoneuron differentiation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | PLURIPOTENT STEM-CELLS | - |
dc.subject | EMBRYOID BODY FORMATION | - |
dc.subject | MOTOR-NEURONS | - |
dc.subject | DIRECTED DIFFERENTIATION | - |
dc.subject | DISEASE | - |
dc.subject | CULTURE | - |
dc.subject | MODELS | - |
dc.subject | BODIES | - |
dc.subject | SYSTEM | - |
dc.title | Uniform-sized neurosphere-mediated motoneuron differentiation in microwell arrays | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sun, Woong | - |
dc.identifier.doi | 10.1002/elps.201700118 | - |
dc.identifier.scopusid | 2-s2.0-85028816364 | - |
dc.identifier.wosid | 000417755800012 | - |
dc.identifier.bibliographicCitation | ELECTROPHORESIS, v.38, no.24, pp.3161 - 3167 | - |
dc.relation.isPartOf | ELECTROPHORESIS | - |
dc.citation.title | ELECTROPHORESIS | - |
dc.citation.volume | 38 | - |
dc.citation.number | 24 | - |
dc.citation.startPage | 3161 | - |
dc.citation.endPage | 3167 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | PLURIPOTENT STEM-CELLS | - |
dc.subject.keywordPlus | EMBRYOID BODY FORMATION | - |
dc.subject.keywordPlus | MOTOR-NEURONS | - |
dc.subject.keywordPlus | DIRECTED DIFFERENTIATION | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | MODELS | - |
dc.subject.keywordPlus | BODIES | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordAuthor | Hydrogel microwell array | - |
dc.subject.keywordAuthor | Motoneuron differentiation | - |
dc.subject.keywordAuthor | Uniform-sized neurosphere | - |
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