Pathway engineering of Enterobacter aerogenes to improve acetoin production by reducing by-products formation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, Ji-Woong | - |
dc.contributor.author | Jung, Hwi-Min | - |
dc.contributor.author | Im, Dae-Kyun | - |
dc.contributor.author | Jung, Moo-Young | - |
dc.contributor.author | Oh, Min-Kyu | - |
dc.date.accessioned | 2021-09-02T23:24:42Z | - |
dc.date.available | 2021-09-02T23:24:42Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2017-11 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/81701 | - |
dc.description.abstract | Enterobacter aerogenes was metabolically engineered for acetoin production. To remove the pathway enzymes that catalyzed the formation of by-products, the three genes encoding a lactate dehydrogenase (IdhA) and two 2,3-butanediol dehydrogenases (budC, and dhaD), respectively, were deleted from the genome. The acetoin production was higher under highly aerobic conditions. However, an extracellular glucose oxidative pathway in E. aerogenes was activated under the aerobic conditions, resulting in the accumulation of 2-ketogluconate. To decrease the accumulation of this by-product, the gene encoding a glucose dehydrogenase (gcd) was also deleted. The resulting strain did not produce 2-ketogluconate but produced significant amounts of acetoin, with concentration reaching 71.7 g/L with 2.87 g/L/h productivity in fed-batch fermentation. This result demonstrated the importance of blocking the glucose oxidative pathway under highly aerobic conditions for acetoin production using E. aerogenes. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | SERRATIA-MARCESCENS H32 | - |
dc.subject | FORMING NADH OXIDASE | - |
dc.subject | ACINETOBACTER-CALCOACETICUS | - |
dc.subject | 2,3-BUTANEDIOL PRODUCTION | - |
dc.subject | GLUCOSE-DEHYDROGENASE | - |
dc.subject | KLEBSIELLA-PNEUMONIAE | - |
dc.subject | EFFICIENT UTILIZATION | - |
dc.subject | SUGARCANE MOLASSES | - |
dc.subject | BACILLUS-SUBTILIS | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.title | Pathway engineering of Enterobacter aerogenes to improve acetoin production by reducing by-products formation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Min-Kyu | - |
dc.identifier.doi | 10.1016/j.enzmictec.2017.07.009 | - |
dc.identifier.scopusid | 2-s2.0-85026448938 | - |
dc.identifier.wosid | 000411538900015 | - |
dc.identifier.bibliographicCitation | ENZYME AND MICROBIAL TECHNOLOGY, v.106, pp.114 - 118 | - |
dc.relation.isPartOf | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.title | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.volume | 106 | - |
dc.citation.startPage | 114 | - |
dc.citation.endPage | 118 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | SERRATIA-MARCESCENS H32 | - |
dc.subject.keywordPlus | FORMING NADH OXIDASE | - |
dc.subject.keywordPlus | ACINETOBACTER-CALCOACETICUS | - |
dc.subject.keywordPlus | 2,3-BUTANEDIOL PRODUCTION | - |
dc.subject.keywordPlus | GLUCOSE-DEHYDROGENASE | - |
dc.subject.keywordPlus | KLEBSIELLA-PNEUMONIAE | - |
dc.subject.keywordPlus | EFFICIENT UTILIZATION | - |
dc.subject.keywordPlus | SUGARCANE MOLASSES | - |
dc.subject.keywordPlus | BACILLUS-SUBTILIS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordAuthor | Acetoin | - |
dc.subject.keywordAuthor | Enterobacter aerogenes | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | 2-Ketogluconate | - |
dc.subject.keywordAuthor | Glucose oxidative pathway | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.