Intracellular ROS levels determine the apoptotic potential of keratinocyte by Quantum Dot via blockade of AKT Phosphorylation
DC Field | Value | Language |
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dc.contributor.author | Lee, Eun Young | - |
dc.contributor.author | Bae, Hyun Cheol | - |
dc.contributor.author | Lee, Hana | - |
dc.contributor.author | Jang, Yeonsue | - |
dc.contributor.author | Park, Yoon-Hee | - |
dc.contributor.author | Kim, Jin Hee | - |
dc.contributor.author | Ryu, Woo-In | - |
dc.contributor.author | Choi, Byeong Hyeok | - |
dc.contributor.author | Kim, Ji Hyun | - |
dc.contributor.author | Jeong, Sang Hoon | - |
dc.contributor.author | Son, Sang Wook | - |
dc.date.accessioned | 2021-09-02T23:56:37Z | - |
dc.date.available | 2021-09-02T23:56:37Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2017-11 | - |
dc.identifier.issn | 0906-6705 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/81817 | - |
dc.description.abstract | Quantum dots (QDs) have shown great potential for biomedical use in a broad range including diagnostic agents. However, the regulatory mechanism of dermal toxicity is poorly understood. In this study, we investigated how QDs-induced apoptosis is regulated in human keratinocytes. We also examined the effect of carboxylic acid-coated QDs (QD 565 and QD 655) on reactive oxygen species (ROS) production and apoptosis-related cellular signalling. The viability of keratinocyte was inhibited by two types of QDs in a concentration-dependent manner. QDs induce ROS production and blockade of AKT phosphorylation. Moreover, the cleavage of AKT-dependent pro-apoptotic proteins such as poly (ADP-ribose) polymerase, caspases-3 and caspases-9 was significantly increased. We also found that a decrease in cellular ROS level by ROS scavenger, N-acetylcysteine (NAC), resulting in the abolishment of QDs-induced AKT de-phosphorylation and cellular apoptosis. Interestingly, QD 655 had a more cytotoxic effect including oxidative stress and AKT-dependent apoptosis than QD 565. In addition, QD 655 had the cytotoxic potential in the human skin equivalent model (HSEM). These data show that QD-induced intracellular ROS levels may be an important parameter in QD-induced apoptosis. These findings from this study indicate that intracellular ROS levels might determine the apoptotic potential of keratinocyte by QD via blockade of AKT phosphorylation. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | SILICA NANOPARTICLES | - |
dc.subject | ENDOTHELIAL-CELLS | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | CANCER CELLS | - |
dc.subject | IN-VITRO | - |
dc.subject | CYTOTOXICITY | - |
dc.subject | PENETRATION | - |
dc.subject | MODULATION | - |
dc.subject | INDUCTION | - |
dc.subject | AUTOPHAGY | - |
dc.title | Intracellular ROS levels determine the apoptotic potential of keratinocyte by Quantum Dot via blockade of AKT Phosphorylation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jeong, Sang Hoon | - |
dc.contributor.affiliatedAuthor | Son, Sang Wook | - |
dc.identifier.doi | 10.1111/exd.13365 | - |
dc.identifier.scopusid | 2-s2.0-85023172064 | - |
dc.identifier.wosid | 000413544700009 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL DERMATOLOGY, v.26, no.11, pp.1046 - 1052 | - |
dc.relation.isPartOf | EXPERIMENTAL DERMATOLOGY | - |
dc.citation.title | EXPERIMENTAL DERMATOLOGY | - |
dc.citation.volume | 26 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1046 | - |
dc.citation.endPage | 1052 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Dermatology | - |
dc.relation.journalWebOfScienceCategory | Dermatology | - |
dc.subject.keywordPlus | SILICA NANOPARTICLES | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | CANCER CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | CYTOTOXICITY | - |
dc.subject.keywordPlus | PENETRATION | - |
dc.subject.keywordPlus | MODULATION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | AUTOPHAGY | - |
dc.subject.keywordAuthor | AKT | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | HSEM | - |
dc.subject.keywordAuthor | keratinocyte | - |
dc.subject.keywordAuthor | quantum dots | - |
dc.subject.keywordAuthor | ROS | - |
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