Design of reactive-end DNA oligomers via incorporation of oxanine into oligonucleotides using terminal deoxynucleotidyl transferase
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, Eui Kyoung | - |
dc.contributor.author | Ki, Mi-Ran | - |
dc.contributor.author | Pack, Seung Pil | - |
dc.date.accessioned | 2021-09-02T23:56:48Z | - |
dc.date.available | 2021-09-02T23:56:48Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2017-11 | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/81818 | - |
dc.description.abstract | Oxanine (Oxa, O), a modified nucleobase, has a novel O-acylisourea structure. Oxa-incorporated oligo-deoxynucleotides (ODNs) are reactive DNA oligomers that permit conjugation with various nucleophilic molecules in an activation-free manner. In this study, we developed a new procedure for enzymatic preparation of reactive end DNA oligomers, using terminal deoxynucleotidyl transferase (TdT), in which a reactive Oxa base is incorporated into the 3'-end of ODNs. One limitation of TdT, an enzyme widely used for end labeling of DNA oligomers, is that it is difficult to control the number of incorporated labels, because it shows template-independent extension with random nucleotides. Notably, TdT showed a rate and efficiency of incorporation of the modified nucleobase, Oxa, different from that of natural bases. We investigated the conditions of TdT-mediated DNA incorporation of Oxa and achieved incorporation of Oxa at the 3'-end of ODNs by optimizing reaction parameters such as temperature and enzyme, cofactor, and substrate concentrations. We also confirmed the reactive functionality of Oxa after incorporation into ODNs by amide bonding conjugation with a polyamine (spermine) under physiological conditions, without need for an additional activation step. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | PROTEIN | - |
dc.subject | OLIGODEOXYNUCLEOTIDE | - |
dc.subject | 2-DEOXYOXANOSINE | - |
dc.subject | NUCLEOTIDES | - |
dc.subject | CELLS | - |
dc.title | Design of reactive-end DNA oligomers via incorporation of oxanine into oligonucleotides using terminal deoxynucleotidyl transferase | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ki, Mi-Ran | - |
dc.contributor.affiliatedAuthor | Pack, Seung Pil | - |
dc.identifier.doi | 10.1016/j.procbio.2017.07.011 | - |
dc.identifier.scopusid | 2-s2.0-85028503443 | - |
dc.identifier.wosid | 000416394500012 | - |
dc.identifier.bibliographicCitation | PROCESS BIOCHEMISTRY, v.62, pp.99 - 105 | - |
dc.relation.isPartOf | PROCESS BIOCHEMISTRY | - |
dc.citation.title | PROCESS BIOCHEMISTRY | - |
dc.citation.volume | 62 | - |
dc.citation.startPage | 99 | - |
dc.citation.endPage | 105 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | OLIGODEOXYNUCLEOTIDE | - |
dc.subject.keywordPlus | 2-DEOXYOXANOSINE | - |
dc.subject.keywordPlus | NUCLEOTIDES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | Oxanine | - |
dc.subject.keywordAuthor | Bioconjugation | - |
dc.subject.keywordAuthor | Terminal deoxynucleotidyl transferase | - |
dc.subject.keywordAuthor | Reactive-end ODNs | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.