Echinicola sediminis sp nov., a marine bacterium isolated from coastal sediment

Abstract

A novel Gram-stain-negative, rod-shaped, gliding bacterium, designated strain 001-Na2(T), was isolated from coastal sediment of the Yellow Sea in Sindu-ri, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. Colonies were dark salmon-coloured, circular, and pulvinate with entire margins. 16S rRNA gene sequence analysis revealed that strain 001-Na2(T) belonged to the genus Echinicola and these species formed a distinct lineage within the family Cyclobacteriaceae. The closest phylogenetic neighbours were 'Echinicola shivajiensis' AK12(T) (98.1% 16S rRNA gene sequence similarity), Echinicola vietnamensis LMG 23754(T) (96.4 %), Echinicola jeungdonensis HMD 3054(T) (96.0 %), Echinicola pacifica KMM 6172(T) (96.0 %) and Echinicola rosea JL 3085(T) (95.8 %). The predominant respiratory quinone was menaquinone MK-7. The major fatty acids were iso-C-15:0, summed feature 3 (comprising C-16:1 omega 7c and/or C-16 :1 omega 6c), C-17:1 omega 6c and summed feature 9 (comprising iso-C-17:1 omega 9c and/or 10-methyl C-16:0). The polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid and three unidentified lipids. The DNA G+C content was 44.6 mol%. DNA-DNA relatedness between strain 001-Na2(T) and 'E. shivajiensis' JCM 17847(T) was 20.8-21.1 %. On the basis of the phenotypic features, and phylogenetic and DNA-DNA hybridization analyses presented, strain 001-Na2(T) (=KCTC 52495(T) = JCM 31786(T) = DSM 103729(T)) is the type strain of a novel species of the genus Echinicola, for which the name Echinicola sediminis sp. nov. is proposed.